0 item(s)
View cart
You have no items in your shopping cart.
$66.00
Sku: Y13242H-50ug
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by March 20, 2026
Description
LN511E8 (Laminin-511 E8 Fragment) is a recombinant laminin fragment derived from laminin-511 that supports strong integrin-mediated cell adhesion. It is widely used as a defined matrix for human pluripotent stem cell attachment and expansion under xeno-free conditions.
Specifications
| Product Details | |
|---|---|
| Code | Y13242H |
| Purity | ≥95%, by SDS-PAGE (under reducing (R)& Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤10 EU/mg by the LAL method. |
| Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of NIH3T3 cells. The ED50 for this effect is ≤10 ng/mL. |
| Accession | O15230&P07942&P11047 |
| Species | Human |
| Expression System | HEK293 |
| Mol. Weight | 141.6 KDa |
| Expression Region | Ala2534-Ala3323&Leu1561-Leu1786&Asn1364-Pro1609 |
| Tag Info | His Tag |
| Formulation | Lyophilized |
| Buffer | Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol, 0.01% Tween 80, pH7.4. |
| Size | 10μg/50μg/1mg |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
- ✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
- ✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
- ✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.
Applications
Hematology
Supports HSC adhesion in vitro.
Stem Cell Research
Maintains pluripotency and adhesion of ESCs/iPSCs in defined culture systems.
Cell Biology
Provides basement membrane cues, regulates adhesion and survival.
Data Figures
Figure 1: Measured in a cell proliferation assay using Nb2-11 cells.
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
