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$336.00
Sku: Y02301-50ug
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by March 20, 2026
Description
IL-6 (Interleukin-6) is a pleiotropic cytokine secreted by various cell types, including T cells, B cells, macrophages, fibroblasts, and endothelial cells. It plays a critical role in immune regulation, inflammatory responses, hematopoiesis, metabolism, and tissue repair. Signaling is mediated through a complex composed of gp130 and the IL-6 receptor (IL-6R), activating pathways such as JAK/STAT, MAPK, and PI3K. In immune cell cultures—including T cells, B cells, and macrophages—IL-6 promotes Th17 cell differentiation (associated with autoimmunity), suppresses regulatory T cell (Treg) function, and stimulates B cell differentiation into plasma cells, thereby enhancing antibody production. In hematopoietic stem cell cultures, IL-6 acts synergistically with other cytokines (e.g., IL-3 and SCF) to promote the proliferation and differentiation of hematopoietic progenitors, particularly along the megakaryocyte and granulocyte lineages.
Specifications
| Product Details | |
|---|---|
| Cat. No. | Y02301 |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤10 EU/mg by the LAL method. |
| Bioactivity | Measured in a cell proliferation assay using M-NSF-60 cells. The ED50 for this effect is 0.2-1 ng/mL. |
| Accession | P05231 |
| Alternative Names | Interleukin-6; IL-6; B-Cell Stimulatory Factor 2; BSF-2; CTL Differentiation Factor; CDF; Hybridoma Growth Factor; Interferon Beta-2; IFN-Beta-2; IL6; IFNB2 |
| Species | Human |
| Expression System | CHO |
| Mol. Weight | 20.8 kDa |
| Expression Region | Val30-Met212 |
| Tag Info | Tag free |
| Form | Lyophilized |
| Buffer | PBS, 5% Mannitol and 0.01% Tween 80, pH7.4. |
| Size | 10μg/50μg/1mg |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at 2°C to 8°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
- ✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
- ✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
- ✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.
Applications
Hematology
Modulates hematopoietic response under inflammation and B cell differentiation.
Immunology
Links innate and adaptive immunity; involved in inflammatory and immune dysregulation.
Stem Cell Research
Used to mimic inflammatory microenvironments, affecting stem cell fate.
Cell Biology
Regulates proliferation, survival, and stress response signaling.
Data Figures
Figure 1: Recombinant Human IL-6 (Cat. No. Y02301) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y02301 SDS-PAGE.
Figure 2: Measured in a cell proliferation assay using M-NFS-60 cells. The ED50 for this effect is 0.2-1 ng/mL.
Figure 3: Highly consistent bioactivity between different production batches of Recombinant Human IL-6. (Cat. No. Y02301).
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
