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$396.00
Sku: Y12821H-50ug
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by March 20, 2026
Description
IL-33 (Interleukin-33) is a cytokine belonging to the IL-1 family and is mainly expressed by epithelial cells, endothelial cells, and fibroblasts. It signals through the ST2 (IL-1RL1) receptor in complex with IL-1RAcP, activating downstream pathways such as NF-κB and MAPK to initiate type 2 immune responses. Functionally, IL-33 acts as an “alarmin” released upon tissue damage, promoting activation of Th2 cells, ILC2s, mast cells, and eosinophils, and inducing the production of IL-5 and IL-13. In *in vitro* cell and organoid models, IL-33 is commonly used to simulate tissue injury–associated inflammation and type 2 immune microenvironments, particularly in studies of allergy, asthma, and epithelial–immune interactions.
Specifications
| Product Details | |
|---|---|
| Cat. No. | Y12821H |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤100 EU/mg by the LAL method. |
| Bioactivity | Measured by its ability to induce IL-8 secretion by HUVEC human umbilic vein endothelial cells. The ED50 for this effect is ≤1.0 ng/mL. |
| Accession | NP_001186569.1 |
| Species | Human |
| Expression System | E. coli |
| Expression Region | Ser112-Thr270 |
| Tag Info | Tag free |
| Form | Lyophilized |
| Buffer | Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH7.4. |
| Size | 10μg/50μg |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at-20°C to-80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
- ✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
- ✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
- ✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.
Applications
Hematology
Influences activation of myeloid and lymphoid immune cells.
Immunology
Acts as an “alarmin” initiating type 2 immune responses.
Cell Biology
Mediates stress and tissue damage signaling.
Data Figures
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
