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$350.00
Sku: GMP-TL505-0050
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by March 13, 2026
Description
The recombinant human Fms-Related Tyrosine Kinase 3 Ligand (FLT3L/Flt3 Ligand) is a high-purity, four-helical bundle cytokine produced in HEK-293 expression systems, typically existing as a noncovalently-linked dimer.
As a pivotal cytokine in the hematopoietic system, FLT3L specifically binds to its receptor FLT3 (CD135/FLK-2/STK-1, a class III receptor tyrosine kinase), activating downstream signaling pathways including RAS-MAPK and PI3K-AKT-MTOR.
This regulates the proliferation, differentiation, and survival of hematopoietic progenitor cells and dendritic cells (DCs), and induces the mobilization of hematopoietic progenitors and stem cells in vivo.
FLT3L critically governs DC development, being particularly essential for plasmacytoid DCs (pDCs), CD8-positive classical DCs (cDCs), and their CD103-positive tissue-resident counterparts.
By synergizing with various growth factors, FLT3L stimulates the expansion and differentiation of diverse blood cell progenitors and enhances anti-tumor immune responses by potentiating the function of DCs, which provide the key link between innate and adaptive immunity by recognizing pathogens and priming specific immune responses, making it highly valuable for applications in cancer immunotherapy and hematopoietic stem cell transplantation.
Its native dimeric configuration preserves full bioactivity.
Specifications
| Product Details | |
|---|---|
| Synonyms | Fms-related tyrosine kinase 3 ligand, FLT3LG, FL, FLT3L, SL cytokine |
| Accession | UniProt P49771-1 |
| Expressed Region | Thr27–Ala181 |
| Tag | C-terminal 6×His-tag |
| Expression System | HEK293 cells |
| Predicted Molecular Weight | 18.4 kDa |
| Purity | > 90% as determined by SDS-PAGE |
| Endotoxin | < 0.1 EU per 1 μg of protein (LAL method) |
| Biological Activity | Measured by OCI-AML5 cell proliferation assay, corresponding to a specific activity of ≥ 5.0 × 105 IU/mg |
| Formulation | Lyophilized from sterile PBS (pH 7.4), typically supplemented with 6% mannitol as a stabilizing protectant |
Features
-
✔
High Purity: This product is purified through advanced chromatographic techniques, achieving a purity level of over 95%, ensuring accurate and consistent experimental results.
-
✔
High Biological Activity: Demonstrated through multiple activity assays, this protein effectively stimulates FLT3 receptor-positive cells, supporting research on cell proliferation and differentiation.
-
✔
Batch Consistency: Produced with stringent quality control measures to ensure high consistency between batches, reducing variability and enhancing the reliability of research outcomes.
- ✔ Animal-Free: Manufactured without the use of animal-derived components, adhering to animal-free standards and broadening its application scope for diverse research needs.
Applications
Hematology
Study the role of FLT3L in the growth and differentiation of hematopoietic stem cells and progenitor cells, providing insights into blood cell development and potential treatments for hematological disorders.
Immunology
Investigate the effects of FLT3L on immune cell development and function, exploring its potential in immunotherapy and immune system modulation.
Cancer Research
Examine the role of FLT3L in cancer cell biology, including tumor growth and immune cell interactions, contributing to the development of novel cancer therapies.
Stem Cell Research
Utilize FLT3L to support the expansion and maintenance of stem cells in culture, advancing research in regenerative medicine and cell-based therapies.
Stability & Storage
- √Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
- √Reconstitution: Dissolve in sterile water for injection, 0.9% NaCl, or PBS (pH7.4), maintaining a final concentration ≥ 100 µg/mL to prevent adsorption.
- √Handling: Aliquot to avoid repeated freeze-thaw cycles.
Instructions for Use
1.Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment prior to use.
2.Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
3.Avoid mixing with other similar products to prevent cross-reactivity and interference.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Follow recommended storage and handling instructions to maintain product stability.
• Support: Contact technical support if assistance is required.
Document
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
