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Sku: GMP-TL903-0050
Recombinant Human Fibronectin (FN) Protein
Description
Fibronectin (FN) is a dimeric glycoprotein with an approximate molecular weight of 230–250 kDa, covalently linked via C-terminal disulfide bonds. Each monomer comprises 12 type I, 2 type II, and 15–17 type III functional domains that orchestrate multidimensional interactions with cell-surface integrins (α5β1/αvβ3) and extracellular matrix components (collagen/heparin) through specific RGD (Arg-Gly-Asp) and CS1 sequences.
Functionally, it participates in coagulation cascades and host defense as a soluble plasma protein, or serves as a cross-linked polymeric core component in tissue engineering scaffolds, regulating cell adhesion, migration, and differentiation via mechanotransduction.
In cell therapy applications, its recombinant fragment (containing Cell-Binding Domain/CBD, Heparin-Binding Domain/HBD, and CS1 sites) acts as a viral transduction enhancer substrate, leveraging spatial co-localization principles to improve lentiviral vector anchoring efficiency in CAR-T production, boosting primary T-cell transduction rates by 3- to 5-fold.
Within 3D mesenchymal stem cell (MSC) culture systems, it activates the FAK-PI3K-Akt signaling axis to preserve stemness and guide neuro/vascular lineage differentiation, significantly enhancing the in vivo survival of tissue-engineered grafts.
Specifications
| Product Details | |
|---|---|
| Synonyms | Cold-insoluble globulin |
| Accession | UniProt P02751-1 |
| Expressed Region | Pro1270–Ser1546 & Ala1721–Thr2016 |
| Tag | N-terminal His tag |
| Expression System | E. coli |
| Predicted Molecular Weight | 68.0 kDa |
| Purity | > 90% as determined by SDS-PAGE |
| Endotoxin | < 0.1 EU per 1 μg of protein (LAL method) |
| Biological Activity | Promotes adhesion of human induced pluripotent stem (hiPS) cells with an ED50 ≤ 3.2 μg/mL |
| Formulation | Liquid formulation in sterile PBS (pH 7.4), typically supplemented with human serum albumin (HSA) as a stabilizing protectant |
Features
-
✔
High Purity: This product is purified using advanced chromatographic techniques, achieving a purity level of over 95%, ensuring accurate and reproducible experimental outcomes.
-
✔
High Biological Activity: Demonstrated through multiple activity assays, this product effectively supports cell adhesion, migration, and proliferation, making it suitable for various cell culture applications.
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✔
Batch Consistency: Produced under stringent quality control measures to ensure consistency between batches, minimizing variability and enhancing the reliability of research results.
- ✔ Animal-Free: Manufactured without the use of animal-derived components, adhering to animal-free standards and expanding its applicability for diverse research needs.
Applications
Cell Adhesion Studies
Investigate the role of fibronectin in cell adhesion and migration, contributing to a deeper understanding of cell-matrix interactions.
Tissue Engineering
Utilize fibronectin in tissue scaffolds and matrices to promote cell attachment and tissue regeneration, advancing the development of regenerative therapies.
Wound Healing Research
Explore the impact of fibronectin on wound healing processes, including tissue repair and remodeling, providing insights into potential therapeutic approaches.
Cancer Research
Study the role of fibronectin in tumor cell behavior and metastasis, offering valuable information for cancer research and treatment strategies.
Stability & Storage
- √ Liquid: Stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
- √ Reconstitution: N/A
- √ Handling: Aliquot to avoid repeated freeze-thaw cycles.
Instructions for Use
Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment prior to use.
Prepare and dilute the solution according to experimental requirements for accurate and reproducible results.
Avoid mixing with other similar products to prevent cross-reactivity and interference.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Follow recommended storage and handling instructions to maintain product stability.
• Support: Contact technical support if assistance is required.
Document
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
