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$11.00
Sku: Y00801-10ug
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by June 06, 2026
Description
EGF (Epidermal Growth Factor) is a small molecular polypeptide composed of 53 amino acid residues and is a member of the EGF family. It binds to specific receptors on the surface of responsive cells, promoting receptor dimerization and phosphorylation of cytoplasmic sites. The activated receptor can interact with at least five different proteins containing distinct signaling sequences to propagate intracellular signals. As a broad-spectrum mitogen, EGF stimulates mitosis in a variety of cells, promotes tissue maturation and regeneration, accelerates capillary formation, and enhances the proliferation of epidermal and endothelial cells. It plays an essential role in regulating cell growth, proliferation, and differentiation.
Specifications
| Product Details | |
|---|---|
| Cat. No. | Y00801 |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤10 EU/mg by the LAL method. |
| Bioactivity | Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast Cells. The ED50 for this effect ≤0.3 ng/mL. |
| Accssion | P01133 |
| Alternative Names | Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone |
| Species | Human |
| Expression System | CHO |
| Mol. Weight | 6.2 kDa |
| Expression Region | Asn971-Arg1023 |
| Tag Info | Tag free |
| Formulation | Lyophilized |
| Buffer | PBS, 5% Mannitol and 0.01% Tween 80, pH7.4. |
| Size | 10μg/50μg/1mg |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
- ✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
- ✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
- ✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.
Applications
Stem Cell Research
Widely used to support proliferation of epithelial, neural, and pluripotent stem cells.
Cell Biology
Promotes cell proliferation, survival, and differentiation via EGFR signaling.
Data Figures
Figure 1: Recombinant Human EGF (Cat. No. Y00801) SDS-PAGE under reducing (R) & Non-reducing condition. The gel was stained with Y00801 SDS-PAGE.
Figure 2: Recombinant Human EGF (Cat. No. Y00801) stimulates proliferation of NIH3T3 cells. The ED50 for this effect ≤0.3 ng/mL. The acivity of Y00801 was equivalent to other competing products.
Figure 3: Highly consistent bioactivity between different production batches of Recombinant Human Transferrin (Cat. No. Y00801).
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
