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$345.00
Sku: TL-787-0050
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by March 20, 2026
Description
As a core member of the FGF family, bFGF selectively binds to FGFR1c/2c/3c/4 receptor subtypes via its β-trefoil structure, activating the Ras-MAPK and PI3K-AKT signaling axes to regulate critical physiological processes, including cell proliferation, migration, differentiation, and angiogenesis.
In cell therapy applications, bFGF serves as an essential component of serum-free stem cell culture systems, sustaining the undifferentiated state of human pluripotent stem cells (hPSCs) through sustained ERK1/2 pathway activation while stabilizing Nanog/Oct4 expression. It further synergizes with BMP inhibitors to prevent spontaneous germ layer differentiation.
This recombinant thermostable human basic fibroblast growth factor (bFGF-HS) is engineered via an AI-driven protein design platform and produced in E. coli as a tag-free protein. While preserving the native heparin-binding domain (residues 128–144) and biological activity, the optimized sequence significantly enhances thermal stability and in vitro potency.
Compared to wild-type bFGF, this mutant exhibits an extended half-life in culture systems, reducing medium replenishment frequency and contamination risks, making it particularly suitable for large-scale stem cell culture, tissue engineering, and regenerative medicine research.
Specifications
| Cytokines Details | |
|---|---|
| Synonyms | FGF2, FGFB, FGF basic, HBGF-2 |
| Accession | UniProt P09038-1 |
| Expressed Region | Gly59–Ser210 with an N-terminal methionine and several site mutations |
| Tag | Tag-free |
| Expression System | E. coli |
| Predicted Molecular Weight | 17.0 kDa |
| Purity | > 90% as determined by SDS-PAGE |
| Endotoxin | < 0.1 EU per 1 μg of protein (LAL method) |
| Biological Activity | Measured by HUVEC cell proliferation assay, corresponding to a specific activity of ≥ 1.0 × 106 IU/mg |
| Formulation | Lyophilized from sterile PBS (pH 7.4), typically supplemented with 6% mannitol as a stabilizing protectant |
Features
-
✔ Heat-Stable Engineered Variant
AI-designed mutations significantly enhance thermal stability while preserving native bFGF activity.
-
✔ Sustained ERK/AKT Signaling
Provides prolonged FGFR activation to support continuous cell proliferation and survival.
-
✔ High Biological Activity
Validated by HUVEC proliferation assay, ensuring reliable potency in serum-free culture systems.
-
✔ Excellent Batch Stability
Consistent performance across batches reduces variability in long-term cell culture workflows.
Applications
Human iPSC Maintenance
Maintains pluripotency by stabilizing Nanog/Oct4 expression and preventing spontaneous differentiation.
Mesenchymal Stem Cell (MSC) Expansion
Promotes robust MSC proliferation and migration in serum-free conditions.
Large-Scale Stem Cell Culture
Extended half-life reduces media change frequency and contamination risk.
Regenerative Medicine & Tissue Engineering Research
Supports angiogenesis, cell migration, and growth factor–dependent tissue models.
Stability & Storage
- √ Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
- √ Reconstitution: Dissolve in sterile water for injection, 0.9% NaCl, or PBS (pH7.4), maintaining a final concentration ≥ 100 µg/mL to prevent adsorption.
- √ Handling: Aliquot to avoid repeated freeze-thaw cycles.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Follow recommended storage and handling instructions to maintain product stability.
• Support: Contact technical support if assistance is required.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
