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$71.50
Sku: TL-901-0010
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by March 13, 2026
Description
Recombinant human basic Fibroblast Growth Factor (bFGF) is a non-glycosylated heparin-binding protein produced in E. coli expression systems, functioning as a core member of the FGF family.
It selectively engages FGFR1c/2c/3c/4 receptor subtypes to activate Ras-MAPK and PI3K-AKT signaling cascades, governing critical physiological processes including cell proliferation, migration, differentiation, and angiogenesis.
In cell therapy applications, bFGF serves as an essential component of serum-free stem cell culture systems, sustaining the undifferentiated state of human pluripotent stem cells (hPSCs) through continuous ERK1/2 pathway activation that stabilizes Nanog/Oct4 pluripotency factors, while synergizing with BMP signaling inhibitors (e.g., Noggin) to block spontaneous germ layer differentiation.
For tissue engineering, its spatiotemporally controlled release strategies direct mesenchymal stem cell (MSC) commitment toward neural/chondrogenic lineages and promote endogenous regenerative niche formation in damaged tissues, significantly enhancing functional integration of neural repair grafts with host neural circuitry.
Specifications
| Parameter | RUO Grade | GMP Grade |
|---|---|---|
| Catalog Number | TL901-0010 | GMP-TL901-0050 |
| Synonyms | FGF2, FGFB, FGF basic, HBGF-2 | FGF2, FGFB, FGF basic, HBGF-2 |
| Accession | UniProt P09038-1 | UniProt P09038-1 |
| Expressed Region | Gly59–Ser210 | Gly59–Ser210 |
| Tag | Tag-free | Tag-free |
| Expression System | E. coli | E. coli |
| Molecular Weight | 17.1 kDa | 17.1 kDa |
| Purity | >90% (SDS-PAGE) | >90% (SDS-PAGE) |
| Endotoxin | <0.1 EU/µg | <0.1 EU/µg |
| Biological Activity | HUVEC proliferation assay, ≥ 1.0 × 106 IU/mg |
HUVEC proliferation assay, ≥ 1.0 × 106 IU/mg |
| Formulation | Lyophilized from PBS (pH 7.4), 6% mannitol | Lyophilized from PBS (pH 7.4), 6% mannitol |
Features
-
✔
Authentic Wild-Type bFGF Activity
Preserves native FGFR binding and downstream ERK/AKT signaling for reliable biological performance.
-
✔
High Biological Potency
Validated by HUVEC proliferation assay, ensuring consistent mitogenic activity in cell culture systems.
-
✔
Tag-Free, E. coli Expression
Non-glycosylated and tag-free design supports compatibility with standard stem cell protocols.
-
✔
Excellent Batch Stability
Consistent purity and endotoxin control reduce variability in routine culture workflows.
Applications
Human iPSC Maintenance
Sustains pluripotency by stabilizing Nanog/Oct4 and preventing spontaneous differentiation.
Mesenchymal Stem Cell (MSC) Culture
Promotes MSC proliferation and migration in serum-free conditions.
Tissue Engineering Research
Supports angiogenesis, cell migration, and lineage commitment in regenerative models.
General Stem Cell & Cell Biology Studies
Ideal for standard protocols requiring well-characterized, literature-consistent bFGF.
Stability & Storage
- √ Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
- √ Reconstitution: Dissolve in sterile water for injection, 0.9% NaCl, or PBS (pH7.4), maintaining a final concentration ≥ 100 µg/mL to prevent adsorption. For 10 μg vial size, dissolve contents in 200 μL of the above solvents.
- √ Handling: Aliquot to avoid repeated freeze-thaw cycles.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Follow recommended storage and handling instructions to maintain product stability.
• Support: Contact technical support if assistance is required.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
