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$350.00
Sku: GMP-TL695-0050
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by March 18, 2026
Description
The recombinant human 4-1BB Ligand-Fc fusion protein is a functional dimeric immunomodulator produced in HEK-293 expression systems, featuring a C-terminal human IgG1 Fc domain to enhance structural stability and receptor clustering efficiency.
As a TNF superfamily member (TNFSF9/CD137L), it activates NF-κB and MAPK signaling pathways through binding to 4-1BB (CD137/TNFRSF9) on T cells, delivering critical costimulatory signals that drive T-cell proliferation, IFN-γ/IL-2 secretion, and expansion of effector memory T-cell subsets, while suppressing activation-induced cell death (AICD) via upregulation of Bcl-xL.
In cell therapy applications, this fusion protein serves as a pivotal component in ex vivo expansion systems, mimicking antigen-presenting cell (APC) costimulatory signals to significantly enhance CAR-T cell clonal expansion and persistence.
It synergizes with CD28 signaling to optimize T-cell metabolic reprogramming and boost effector functions of tumor-infiltrating lymphocytes (TILs), providing functional support for solid tumor immunotherapy.
The Fc domain design not only extends serum half-life but also ensures high-affinity trimeric engagement with 4-1BB receptors through spatial conformation optimization, enabling sustained signal activation.
Specifications
| Parameter | Specification | Results |
|---|---|---|
| Appearance | Freeze-dried preparation, the form should be white loose body | Compliance |
| Identity | The electrophoretic bands should be consistent with the molecular weight of the product | Compliance |
| Visible Particle | Should be no visible particle | Compliance |
| Moisture | ≤4.0% | 1.4% |
| pH | 6.5~7.5 | 7.3 |
| Purity | >90.0% | Compliance |
| Endotoxin | <0.1EU/μg | <0.1EU/μg |
| Mycoplasma | Should be negative | Negative |
| Sterility | No growth | No growth |
| Biological Activity | Each hole is covered by 100μL Recombinant human 4-1BB (His tag) protein with a concentration of 1μg/mL; the ability of 4-1BB Ligand (Fc tag) to bind to 4-1BB (His tag) protein was determined by ELISA, ED50 ≤ 50ng/mL | 20.53ng/mL |
Features
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Mammalian-Expressed Fc Fusion Design
Recombinant human 4-1BB Ligand (Ala50–Glu254) expressed in HEK293 cells with a C-terminal human IgG1 Fc fragment, forming a dimeric fusion protein with a predicted molecular weight of 51.0 kDa.
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✔
High Purity and Identity Verification
Purity >90% as determined by SDS-PAGE, with electrophoretic bands consistent with the expected molecular weight and no visible particles detected.
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Low Endotoxin and Sterility Assurance
Endotoxin level <0.1 EU per 1 µg protein (LAL assay), mycoplasma negative, and sterility confirmed with no microbial growth.
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✔
Validated 4-1BB Binding Activity
Binds immobilized recombinant human 4-1BB/His Tag (1 µg/mL, 100 µL/well) in functional ELISA with ED₅₀ ≤50 ng/mL (measured 20.53 ng/mL).
Applications
T Cell Costimulatory Activation
Engages 4-1BB (CD137/TNFRSF9) on T cells to activate NF-κB and MAPK pathways, promoting T-cell proliferation and IFN-γ/IL-2 secretion.
CAR-T Ex Vivo Expansion Support
Functions as a costimulatory component in ex vivo expansion systems to enhance CAR-T cell clonal expansion and persistence.
Effector Memory T Cell Modulation
Drives expansion of effector memory T-cell subsets and suppresses activation-induced cell death via Bcl-xL upregulation.
TIL Functional Enhancement Research
Synergizes with CD28 signaling to optimize T-cell metabolic reprogramming and enhance effector functions of tumor-infiltrating lymphocytes.
Stability & Storage
- Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
- Reconstitution: Dissolve in sterile Water for Injection, 0.9% NaCl, or PBS (pH 7.4), maintaining final concentration ≥100 µg/mL to prevent adsorption.
- Handling: Aliquot to avoid repeated freeze-thaw cycles.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Prepare solutions using sterile technique. Avoid microbial contamination to maintain activity.
• Storage: Follow recommended storage conditions. Repeated freeze–thaw cycles may reduce performance.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
