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Description
Precast-GLgel Hepes SDS-PAGE 15% (10-Well) is a high-concentration, ready-to-use protein electrophoresis gel designed for precise separation of small to medium molecular weight proteins. Made with an innovative glass plate structure, it minimizes non-specific protein adsorption and provides sharper, clearer bands.
The 15% HEPES-based precast gel is pre-prepared and supplied with compatible running buffer, allowing users to load samples directly without making gels by hand. This design improves experimental efficiency and ensures superior consistency.
Due to its high acrylamide concentration and stable HEPES buffer system, this gel is also suitable for separating membrane proteins and membrane protein complexes, which often require a tighter gel matrix for clear resolution.
Specification
| Full name | Precast-GLgel Hepes SDS-PAGE 15% 10-Well |
| Package | 10 pcs |
| Components | 10 pcs gel, 10 pcs SDS-PAGE running buffer |
| Gel size | 8 × 7.4 × 0.15 cm |
| Plate size | 9.8 × 8.4 × 0.41 cm |
| Wells | 10 wells |
| Buffer system | HEPES buffer system |
| Maximum sample loading volume | 60 µL |
| Recommended sample loading volume | 30 µL |
Buffer
| Buffer | Hepes Running Buffer for SDS-PAGE (Powder) |
| Specification | 10*500 mL |
| Introduction | Hepes Running buffer is formulated for separation of proteins in their denaturing or nondenaturing state on Hepes-Tris gels. This product is available in powder form, a total of 10 packs, each pack can prepare 500 mL of 1× working solution. |
| Protocol (for reference only) |
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| Notes |
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| Storage | Store at room temperature, valid for 2 years. |
Features
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Glass plate construction effectively reduces non-specific protein adsorption and delivers sharper, clearer bands.
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Automated gel filling technology ensures stability and excellent batch-to-batch reproducibility.
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High-concentration (15%) gel provides strong resolving power for small to medium molecular weight proteins.
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Suitable for membrane proteins and membrane protein complexes, which benefit from the tighter gel matrix and stable HEPES buffer conditions.
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SDS-free gel matrix, compatible with both denaturing electrophoresis (SDS-PAGE) and non-denaturing electrophoresis (Native-PAGE).
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Precast and ready to use, eliminating gel preparation steps and saving significant laboratory time.
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Long shelf life and high compatibility, stored at 2–8°C.
Applications
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SDS-PAGE analysis
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Separation of small to medium molecular weight proteins
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Electrophoresis of membrane proteins and membrane protein complexes
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Native-PAGE (non-denaturing electrophoresis)
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Western blot (WB) protein analysis
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Experiments requiring a high-percentage 15% gel for fine resolution
Storage
2~8°C, do not freeze
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
