Precast-Gel Hepes Native-PAGE 7.5% 10Well

Description

Precast-GLgel Hepes Native-PAGE 7.5% (10-Well) is a ready-to-use, non-denaturing gel designed for the analysis of proteins in their native, biologically active state. Constructed with an innovative glass plate design, it minimizes non-specific protein adsorption and delivers sharp, clear protein bands.

This 7.5% HEPES-based precast gel comes fully prepared with matched Native-PAGE running buffer, allowing direct sample loading without manual gel preparation. The 7.5% concentration is optimized for resolving larger molecular weight native proteins, including membrane proteins and native protein complexes, maintaining structural integrity while enabling gentle separation.

Specification

Features

• Glass plate construction minimizes non-specific protein adsorption, producing sharper and clearer protein bands.

• Automatic gel filling technology ensures consistent quality and excellent batch-to-batch reproducibility.

• 7.5% acrylamide concentration provides optimal resolution for larger molecular weight native proteins.

• Suitable for membrane proteins and membrane protein complexes, preserving structural integrity under non-denaturing HEPES conditions.

• Supports separation of native protein complexes, including oligomers and multi-subunit assemblies.

• SDS-free gel matrix, ideal for true Native-PAGE studies and downstream functional analyses.

• Ready-to-use precast format eliminates manual gel preparation, saving significant laboratory time.

• Long shelf life with convenient storage at 2–8°C.

Applications

• Native-PAGE analysis of proteins in non-denatured form

• Separation of larger molecular weight native proteins

• Electrophoresis of membrane proteins and membrane protein complexes

• Analysis of native oligomers and protein complexes

• Western blot (WB) after native separation

• Experiments requiring a 7.5% native gel

Storage

2~8°C, do not freeze