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Description
Precast-GLgel Hepes Native-PAGE 4–20% (10-Well) is a ready-to-use, non-denaturing gradient gel designed for analyzing proteins in their native, biologically active conformations. The gel is built with an innovative glass plate design that minimizes non-specific protein adsorption and provides sharper, clearer native protein bands.
This 4–20% HEPES-based precast gel is supplied fully prepared with matched running buffer, allowing direct sample loading without manual gel preparation. The wide gradient range makes it suitable for resolving a broad spectrum of native protein sizes, including large molecular weight protein complexes and membrane protein complexes, which require gentle electrophoresis conditions to preserve native structure.
Specification
| Full name | Precast-GLgel Hepes Native-PAGE 4–20% 10-Well |
| Package | 10 pcs |
| Components | 10 pcs gel, 10 pcs Native-PAGE running buffer |
| Gel size | 8 × 7.4 × 0.15 cm |
| Plate size | 9.8 × 8.4 × 0.41 cm |
| Wells | 10 wells |
| Buffer system | HEPES buffer system |
| Maximum sample loading volume | 60 µL |
| Recommended sample loading volume | 30 µL |
Features
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Glass plate construction minimizes non-specific protein adsorption, producing sharper and clearer native bands.
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Automated gel filling technology ensures excellent stability and reproducibility across batches.
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4–20% gradient gel provides broad-range separation for diverse native protein sizes.
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Ideal for large molecular weight native proteins and protein complexes, which benefit from the gentle separation conditions of the HEPES system.
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Well-suited for membrane proteins and membrane protein complexes, supporting analysis while preserving native conformation.
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SDS-free gel matrix, fully compatible with non-denaturing electrophoresis (Native-PAGE) and downstream native studies.
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Precast and ready to use, eliminating manual gel preparation and significantly reducing experiment time.
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Long shelf life, stored conveniently at 2–8°C.
Applications
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Native-PAGE analysis of proteins in non-denatured form
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Separation of large molecular weight native proteins
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Electrophoresis of membrane proteins and membrane protein complexes
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Analysis of native protein complexes (e.g., oligomers, assemblies)
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Western blot (WB) after native separation
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Experiments requiring a 4–20% native gradient gel
Storage
2~8°C, do not freeze
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
