Near-infrared (NIR) Duo Colour Prestained Protein Marker (10-250 kDa)

Description

In SDS-PAGE and Western blot workflows, researchers need a reliable protein molecular weight reference that can be seen during electrophoresis, helps confirm transfer performance, and supports quick estimation of target protein size. Poor band visibility, uncertain transfer efficiency, or mismatched molecular weight references can make gel interpretation and blot analysis less consistent.

Multicolor Prestained Protein Ladder WJ101, WJ102, WJ103, and WJ106 are ready-to-use prestained protein molecular weight markers designed for protein electrophoresis, SDS-PAGE analysis, and Western blot transfer monitoring. These protein ladders include colored reference bands to help monitor protein separation in real time and estimate the approximate molecular weight of target proteins after electrophoresis or membrane transfer.

WJ101 contains 9 prestained protein bands from 15 kDa to 150 kDa: 15, 20, 25, 35, 40, 50, 70, 100, and 150 kDa. WJ102, WJ103, and WJ106 contain 11 prestained protein bands from 10 kDa to 250 kDa: 10, 15, 20, 25, 35, 40, 50, 70, 100, 150, and 250 kDa. The 25 kDa and 70 kDa bands are coupled with red or green dyes for easy identification during gel running and Western blot workflows.

The ladder is supplied in loading buffer and can be loaded directly onto gels without heating or boiling. WJ106 is recommended for workflows involving near-infrared fluorescence imaging systems.

Specifications

Buffer Composition

62.5 mM Tris-HCl, pH 7.5 at 25°C; 1 mM EDTA; 2% (W/V) SDS; 10 mM DTT; 33% (W/V) glycerol.

Product Diagram

Applications

• Used as a prestained protein ladder for SDS-PAGE protein electrophoresis.

• Supports approximate protein molecular weight determination from 15–150 kDa or 10–250 kDa, depending on the selected model.

• Helps monitor protein separation during SDS-polyacrylamide gel electrophoresis.

• Useful as a Western blot protein ladder for checking membrane transfer progress.

• Supports evaluation of Western transfer efficiency on common blotting membranes.

• Provides colored reference bands for real-time gel orientation and band identification.

• Suitable for routine protein analysis, protein expression checks, and target protein size estimation.

• Can be used with Tris-Glycine and other common electrophoresis buffer systems, with migration differences considered during interpretation.

• WJ106 is suitable for near-infrared fluorescence imaging workflows.

• Recommended for research laboratories requiring a ready-to-use protein molecular weight marker without heating or boiling.

Operating Instructions

1. Before loading: Allow the sample to sit at room temperature for a few minutes. After thawing, gently mix by swirling to ensure the solution is homogeneous.

2. Loading: Load an appropriate volume into the gel wells. The recommended loading volume is 2–5 μL per well and may be adjusted according to gel format and visualization needs.

3. Storage after use: Seal the tube after use and store at 4°C or -20°C.

Note: For a 5 μL loading volume, the protein content of each band in WJ101, WJ102, WJ103, and WJ106 is approximately 1–3 μg.

Molecular Weights Under Different Buffer Conditions

The apparent molecular weight of prestained protein markers may vary under different electrophoresis buffer systems. Use the chart below as a reference when interpreting band migration in different gel and running buffer conditions.

Precautions

• This product includes loading buffer and should be used directly without heating or boiling.

• To avoid repeated freeze-thaw cycles and contamination, aliquot the product and store at -20°C.

• Prestained protein molecular weight markers are intended for approximate molecular weight reference only, with batch-to-batch variation of about 5%.

• For near-infrared fluorescence imaging systems, please select WJ106.

• In low-concentration gels, low molecular weight bands may co-migrate with the front and may not be fully resolved.

• The migration rate of prestained proteins may vary in different gels and buffer systems. Calibration with unstained protein molecular weight markers is recommended when more accurate sizing is required.

• Protein markers may exhibit non-specific binding to antibodies. Optimization may include reducing antibody concentration, shortening incubation time, or decreasing the amount of protein marker used.

• Wear a lab coat and disposable gloves during handling.

• For research use only.

Storage

This product requires shipping with ice packs. Avoid repeated freeze-thaw cycles. Shelf life is 3 months at 4°C and 24 months at -20°C.

FAQ

Documents

Multicolor Prestained Protein Ladder User Manual