NC Blotting Membranes

Description

Biofargo Nitrocellulose (NC) Transfer Membranes are high-performance membranes designed for reliable protein and nucleic acid detection across a wide range of blotting applications. Made from 100% pure nitrocellulose, these membranes offer strong binding capacity, high sensitivity, and excellent signal-to-noise ratios. They require no pre-activation, ensuring easy handling and streamlined workflows. Optimized for Western blotting and suitable for Southern, Northern, and dot/slot blotting, the membranes deliver high resolution, low background, and consistent experimental results. Available in multiple pore sizes, they support accurate transfer of proteins of different molecular weights.

Specifications

Features

• 100% Pure Nitrocellulose — Ensures consistent quality, uniform pore structure, and strong affinity for proteins and nucleic acids.

• High Binding Capacity & Sensitivity — Provides strong adsorption of target molecules for clear and reliable detection.

• No Pre-activation Required — Ready to use without methanol or additional activation steps, simplifying blotting workflows.

• Low Background & Excellent Signal-to-Noise Ratio — Produces clean, high-contrast bands ideal for chemiluminescent, chromogenic, and fluorescent detection.

• Compatible With Multiple Detection Methods — Performs well with a broad range of assay systems, including colorimetric, chemiluminescent, and fluorescence-based detection.

• Supports Long-lasting Protein Activity — Derived from natural nitrocellulose materials that help maintain immobilized protein antigenicity.

• Optimized Pore Size Options — 0.1 µm, 0.22 µm, and 0.45 µm membranes designed to match different molecular weight targets and transfer requirements.

• High Resolution and Reproducibility — Ensures uniform transfer quality across the entire membrane for consistent experimental outcomes.

Applications

• Western Blotting

• Southern Blotting

• Northern Blotting

• Dot/Slot Blotting

FAQ

• Q1. How do I choose the right pore size (0.1, 0.22 or 0.45 µm)?

For small proteins or peptides (e.g. below ~10–20 kDa), or when high detection sensitivity for low-molecular-weight targets is needed, choose the smaller-pore membranes (0.1 or 0.22 µm).

For majority of proteins (especially > 20 kDa) or general-purpose blotting, 0.45 µm is the most versatile and widely used.

• Q2. Do I need to pre-activate (pretreat) the membrane before transfer?

  No — because your NC membranes are 100% pure nitrocellulose, they do not require methanol or ethanol pre-wetting. This simplifies workflow and reduces handling time.

• Q3. Which detection methods are compatible with the membrane?

  This NC membrane supports chemiluminescent, chromogenic (colorimetric), and fluorescent detection methods — giving users flexibility depending on the assay and available equipment.

• Q4. Can I store a blot after transfer for future re-probing or analysis?

  Yes — after transfer and drying (or in buffer), the immobilized proteins or nucleic acids remain stably bound. However, because NC is more fragile than some alternative membranes, handle carefully to avoid mechanical damage.

• Q5. What if I see high background or non-specific bands in my blot?

  Possible causes include incomplete blocking, overly concentrated antibodies, or overly long exposure times. Using optimal blocking buffer, proper antibody dilution, and minimizing membrane dryness during handling will help reduce background.

• Q6. Is this membrane suitable for both protein and nucleic-acid blotting (e.g. DNA/RNA)?

  Yes — the membrane’s high binding affinity and broad compatibility make it suitable for protein Western blotting and nucleic-acid blotting (Southern, Northern) as well as dot or slot blot assays.

• Q7. What are advantages of NC membrane vs other membranes such as PVDF?

No pre-activation needed, simplifying the experiment workflow.

Typically lower background and good performance in chemiluminescent and fluorescent detection.

For many mid- to low-molecular-weight proteins, NC offers excellent sensitivity and clear results.

Often more cost-effective for routine blotting applications.

• Q8. Are there limitations / things to watch out for with NC membranes?

  Yes — compared with more durable membranes (e.g. PVDF), NC membranes tend to be more brittle and require careful handling; if repeated stripping/reprobing or exposure to harsh conditions is needed, other membranes may perform better.