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Mouse colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31931
$198.00
Sku: RP650-10UG
Bcakground
Macrophage Colony Stimulating Factor(M-CSF),also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF protein is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human M-CSF, respectively (9, 10). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.
Reference
1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
4. Ryan, G.R. et al. (2001) Blood 98:74.
5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
6. DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389.
7 Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706.
Specifications
| Synonyms | colony stimulating factor 1 (macrophage); CSF1; CSF-1; MCSF; M-CSF |
| Accession # | P07141 |
| Source | Human embryonic kidney cell, HEK293-derived Mouse M-CSF protein |
| Lys33-Glu262 | |
| Predicted Moleucular weight | 26 kDa(Monomer) |
| Form/Structure | Dimer in solution |
Components and Storage
| Formulation | Solution protein |
| Dissolved in sterile PBS buffer. | |
| This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening. | |
| Storage and Stability | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| It is recommended that the protein be aliquoted for optimal storage. | |
| 12 months from date of receipt, -20 to -70 °C as supplied. | |
| Shipping | Shipping with dry ice. |
Quality
| Purity | > 95%, determined by SDS-PAGE. |
| Endotoxin Level | <0.010 EU per 1 ug of the protein by the LAL method. |
| Activity | Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. |
| The EC50 for this effect is 0.2-1.5 ng/mL. |
SDS-PAGE
Gel filtration
Bioactivity
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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