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Mouse B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra
$198.00
Sku: RP580-10UG
Bcakground
Interleukin-4 (IL-4) , also known as B cell-stimulatory factor-1, is a monomeric, approximately Th2 cytokine that shows pleiotropic effects during immune responses (1-4). Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain. The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naive CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10 - 13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).
Reference
1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
3. Lee, F. et al. (1986) Proc. Natl. Acad. Sci. 83:2061.
4. Noma, Y. et al. (1986) Nature 319:640.
5. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
6. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
7. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
8. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
9. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
10. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
11. Corthay, A. (2006) Scand. J. Immunol. 64:93.
12. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
13. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
14. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Specifications
| Synonyms | B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4 |
| Accession # | P07750 |
| Source | Human embryonic kidney cell, HEK293-derived Mouse IL-4 protein |
| His23-Ser140 | |
| Predicted Moleucular weight | 13.4 kDa |
Components and Storage
| Formulation | Solution protein |
| Dissolved in PBS buffer. | |
| This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening. | |
| Storage and Stability | Avoid repeated freeze-thaw cycles. |
| It is recommended that the protein be aliquoted for optimal storage. | |
| 12 months from date of receipt, -20 to -70 °C as supplied. | |
| Shipping | Shipping with dry ice. |
Quality
| Purity | > 95%, determined by SDS-PAGE. |
| Endotoxin Level | <0.010 EU per 1 ug of the protein by the LAL method. |
| Activity | Measured in a cell proliferation assay using HT-2 mouse T cells. |
| The EC50 for this effect is 0.2-1.0 ng/mL. |
SDS-PAGE
Gel filtration
Bioactivity
Documents
When can I expect my order to ship?
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We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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