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CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A
$160.00
Sku: RP630-10UG
Bcakground
Interleukin-17A(IL-17A) , also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature mouse IL-17A shares 61% and 89% amino acid sequence identity with human and rat IL-17A, respectively (3, 4). IL-17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide-linked homodimers as well as disulfide-linked heterodimers with IL-17F (5, 6). IL-17A exerts its effects through the transmembrane IL-17RA in complex with IL-17RC or IL-17RD (7, 8). Both IL-17RA and IL-17RC are required for responsiveness to heterodimeric IL-17A/F (7). IL-17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9-12). IL-17A/F likewise induces neutrophil migration, but IL-17F does not (11). IL-17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF-alpha induced shock (Fossiez, 14). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL -17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (15,16). IL-17A has been shown to exert either tumorigenic or anti-tumor effects (17, 18).
Reference
1. Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
2. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
3. Yao, Z. et al. (1996) Gene 168:223.
4. Rouvier, E. et al. (1993) J. Immunol. 150:5445.
5. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
6. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
7. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
8. Rong, Z. et al. (2009) Cell Res. 19:208.
9. Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
10. Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
11. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
12. O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
13. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
14. Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
15. Hsu, H. et al. (2008) Nat. Immunol. 9:166.
16. Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
17. Wang, L. et al. (2009) J. Exp. Med. 206:1457.
18. Kryczek, I. et al. (2009) Blood 114:357.
Specifications
| Synonyms | IL17; IL-17; IL17A; IL-17A; CTLA8; CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8 |
| Accession # | Q62386 |
| Source | Human embryonic kidney cell, HEK293-derived mouse IL-17/IL-17A protein |
| Ala26-Ala158 | |
| Predicted Moleucular weight | 15.0 kDa |
Components and Storage
| Formulation | Solution protein |
| Dissolved in sterile PBS buffer. | |
| This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening | |
| Storage and Stability | Avoid repeated freeze-thaw cycles. |
| It is recommended that the protein be aliquoted for optimal storage | |
| 12 months from date of receipt, -20 to -70 °C as supplied. | |
| Shipping | Shipping with dry ice |
Quality
| Purity | > 95%, determined by SDS-PAGE |
| Endotoxin Level | <0.010 EU per 1 ug of the protein by the LAL method |
| Activity | Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells |
| The EC50 for this effect is 0.12-1.25 ng/mL. |
SDS-PAGE
Bioactivity
Documents
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