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Bcakground
Interferon-alpha 2 (IFN alpha -2) is one of 14 subtypes within the IFN-alpha class of Type I Interferons (1). The members of the IFN-alpha class, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1, 3, 4). IFN-alpha class members signal through a common cell surface receptor complex composed of IFN-alpha R2 and IFN-alpha R1 subunits (3). As the first highly active IFN to be cloned and produced, IFN alpha -2 has become the prototypic IFN for academic and pharmaceutical research (2). The mature extracellular domain (ECD) of mouse IFN alpha -2 shares 60% and 83% aa sequence identity with human and rat, respectively. Murine IFN-alpha 2 can eliminate cardiac viral load and protect cardiomyocytes from injury in animals infected with coxsackievirus B3 (CVB3) (5). IFN alpha -2 derived mutants with reduced IFNR2 binding inhibited HIV replication and mutants with more IFNAR1 binding potentiated antiviral activity (6).
Reference
1. Pestka, S. (2007) J Biol Chem. 282:20047.
2. Paul, F. et al. (2015) Gene. 567(2):132.
3. Oritani, K. et al. (2001). Cytokine & Growth Factor Reviews, 12:337.
4. Pesch, V. et al. (2004). Journal of Virology, 78:8219.
5. Wang, Y.X. et al. (2007) Am J Physiol Heart Circ Physiol. 293:H69.
6. Schlaepfer, E. et al. (2019) Am Soc for Microbiology 4:e00637.
Specifications
| Synonyms | alpha-2a interferon; Ifa2; IFNA; IFNA2; IFNA2a; IFNA2b; IFNA2c; IFNAA; IFNalpha 2; IFN-alpha 2 |
| Accession # | P01573 |
| Source | Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 2/IFNA2 protein |
| Cys24-Glu190 | |
| Predicted Moleucular weight | 19.4 kDa |
Components and Storage
| Formulation | Solution protein |
| Dissolved in sterile PBS buffer. | |
| This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening | |
| Storage and Stability | Avoid repeated freeze-thaw cycles. |
| It is recommended that the protein be aliquoted for optimal storage. | |
| 12 months from date of receipt, -20 to -70 °C as supplied. | |
| Shipping | Shipping with dry ice |
Quality
| Purity | > 95%, determined by SDS-PAGE |
| Endotoxin Level | <0.010 EU per 1 ug of the protein by the LAL method |
| Activity | Measured in an anti-viral assay using L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus |
| The EC50 for this effect is 20-200 pg/mL. |
SDS-PAGE
Bioactivity
Documents
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