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$3,200.00
Sku: H-6013IM.RFP
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by October 08, 2026
Description
Immortalized RFP-Expressing Human Primary Lung Fibroblasts are immortalized fibroblasts isolated from human lung tissue and stably labeled with red fluorescent protein (RFP). Cells are expanded in gelatin-coated tissue culture flasks, harvested at passage 2–3 and cryopreserved at 0.5 × 106 cells per vial. They show typical spindle-shaped fibroblast morphology and are characterized by fibronectin immunofluorescence.
Red fluorescent protein (RFP) is introduced by lentiviral transduction, giving stable, bright red fluorescence that is retained through cell division. Cells can be tracked live by fluorescence microscopy, high-content imaging or flow cytometry with a standard RFP/TRITC filter set, without antibody staining. Immortalization extends expansion to more than 10 passages, giving a renewable, reporter-labeled lung fibroblast population for long-term and repeat experiments.
Suggested medium: Complete Fibroblast Medium with Supplement Kit, 500 mL.
Specifications
| Parameter | Specification |
| Product Name | Immortalized RFP-Expressing Human Primary Lung Fibroblasts |
| Catalog Number | H-6013IM.RFP |
| Species | Human |
| Tissue | Lung |
| Cell Type | Fibroblast — immortalized |
| Reporter | Red fluorescent protein (RFP), lentiviral, stable |
| Immortalization | SV40 large T antigen or hTERT over-expression |
| Format | Cryopreserved vial, 0.5 × 106 cells/mL |
| Passage at Freezing | P2–P3 |
| Expansion | More than 10 passages at 1:2 |
| Quality Control | Negative for HIV-1, hepatitis B, hepatitis C, mycoplasma, bacteria, yeast and fungi. |
| Culture Vessel | Gelatin-coated tissue culture flask |
| Recommended Medium | Complete Fibroblast Medium with Supplement Kit (BF-41875525) |
| Intended Use | For research use only |
Features
Stable RFP expression from lentiviral transduction — no staining needed to identify fibroblasts in mixed cultures.
Immortalized for extended expansion and consistent results across repeat experiments.
Pairs with GFP-labeled epithelial, endothelial or tumor cells for two-color co-culture imaging.
Tested negative for HIV-1, hepatitis B, hepatitis C, mycoplasma, bacteria, yeast and fungi.
Matched medium available for a complete, ready-to-culture system.
Applications
Pulmonary Fibrosis Research: TGF-β-induced myofibroblast differentiation, collagen deposition and anti-fibrotic compound screening.
Co-culture & Tumor Microenvironment: Track fibroblasts alongside GFP-labeled lung epithelial or cancer cells in 2D, 3D spheroid and organoid models.
Cell Migration & Wound Healing: Live imaging of fibroblast migration and invasion assays.
Drug Screening: Fluorescence-based readouts of fibroblast proliferation and survival in high-content assays.
Storage & Shipping
Shipped frozen on dry ice by overnight delivery. Live-cell (flask) shipment is available on request.
On arrival, transfer vials immediately to liquid nitrogen (vapor phase, below −150 °C) until use.
Do not store primary cells at −20 °C or −80 °C.
Avoid repeated freeze–thaw cycles.
Frequently Asked Questions (FAQs)
Common questions about Immortalized RFP-Expressing Human Primary Lung Fibroblasts.
How is the RFP label introduced, and is it stable?
RFP is introduced by lentiviral transduction, which integrates the reporter into the genome. Expression is stable and passed on through cell division.
Which filter set should I use to image these cells?
A standard RFP or TRITC filter set (yellow-green excitation, red emission) is suitable for microscopy, high-content imaging and flow cytometry.
What medium and culture vessel do you recommend?
Use the Complete Fibroblast Medium with Supplement Kit, 500 mL and gelatin-coated tissue culture flasks (0.1% gelatin) for best attachment and growth.
How many passages can the cells be expanded?
More than 10 passages at a 1:2 split ratio.
Should I choose primary or immortalized cells?
Primary cells best reflect native lung fibroblast biology but have a limited lifespan. Immortalized cells provide a renewable supply for repeat and long-term experiments. Many labs use primary cells for key experiments and immortalized cells for assay development and screening.
How should the cells be stored on arrival?
Transfer vials to liquid nitrogen immediately. Do not store at −20 °C or −80 °C, and avoid repeated freeze–thaw cycles.
Are these cells for clinical use?
No. They are for research use only and are not for diagnostic or therapeutic use.
Biosafety
Handle all human-derived cells as potentially infectious and follow BSL-2 practices as described in Biosafety in Microbiological and Biomedical Laboratories (BMBL).
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
