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Bcakground
Interleukin-7 (IL-7) , is a 25 kDa cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival (1-4). Human IL-7 cDNA encodes 177 amino acids (aa) that include a 25 aa signal peptide (3). Human IL-7 shares approximately 60-63% aa sequence identity with mouse, rat, canine and feline IL-7, and 72-76% with equine, bovine, ovine, porcine, feline and canine IL-7. Human and mouse IL-7 exhibit cross-species activity (2, 3). IL-7 protein is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone marrow, and intestines (1, 2, 5). Circulating IL-7 protein is limiting in healthy animals, but increases during lymphopenia (1, 6). IL-7 signals through a complex of the IL-7 Receptor alpha subunit (IL-7 R alpha, also known as CD127) with the common gamma chain ( gamma c) (1).IL-7 contributes to the maintenance of all naive and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2 (7-9). It is required for optimal T cell-dendritic cell interaction (6). IL-7 is expressed early in B cell development prior to the appearance of surface IgM (1, 5, 7). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development (4, 7, 10, 11). However, IL-7 functions in both mouse and human pro-B cells to suppress premature Ig light chain recombination during proliferative growth (12, 13).
Reference
1. Sasson, S.C. et al. (2006) Curr. Drug Targets 7:1571.
2. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
3. Goodwin, R.G. et al. (1990) Proc. Natl. Acad. Sci. USA 86:302.
4. Namen, A.E. et al. (1988) Nature 333:571.
5. Shalapour, S. et al. (2012) PLoS ONE 7: e31939.
6. Saini, M. et al. (2009) Blood 113:5793.
7. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
8. Seddon, B. et al. (2003) Nat. Immunol. 4:680.
9. Schluns, K.S. et al. (2000) Nat. Immunol. 5:426.
10. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
11. Pribyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
12. Johnson, K. et al. (2012) J. Immunol. 188:6084.
13. Nodland, S.E. et al. (2011) Blood 118:2116.
Specifications
| Synonyms | Human IL7; hIL-7; Interleukin-7; interleukin 7; Lymphopoietin-1; PBGF |
| Accession # | P13232 |
| Source | Human embryonic kidney cell, HEK293-derived human IL-7 protein |
| Asp26-His177 | |
| Predicted Moleucular weight | 17.4 kDa |
Components and Storage
| Formulation | Solution protein. |
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Dissolved in sterile PBS buffer. |
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This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening. |
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| Storage and Stability | Avoid repeated freeze-thaw cycles. |
| It is recommended that the protein be aliquoted for optimal storage. | |
| 12 months from date of receipt, -20 to -70 °C as supplied. | |
| Shipping | Shipping with dry ice. |
Quality
| Purity | > 95%, determined by SDS-PAGE. |
| Endotoxin Level | <0.010 EU per 1 ug of the protein by the LAL method. |
| Activity | Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). |
| The EC50 for this effect is 0.1-0.5 ng/mL. |
SDS-PAGE
Gel filtration
Bioactivity
Documents
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