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Sku: 1402430

Description

SHENTEK® Human IFN-γ ELISA Kit is suitable for the quantitation of human interferon-gamma (IFN-γ), for example, in potency assays of CAR-T cells.

Overview

This kit is based on a solid-phase enzyme-linked immunosorbent assay (ELISA) using a double-antibody sandwich technique to detect human IFN-γ. An antibody specific to human IFN-γ is employed in the assay to capture any IFN-γ present in the sample. Both the calibration standards and test samples are simultaneously added to a microtiter plate coated with an affinity-purified capture antibody, followed by incubation and washing. A biotinylated antibody is then added to bind human IFN-γ, which subsequently reacts with streptavidin-labeled HRP (horseradish peroxidase). TMB (3,3′,5,5′-tetramethylbenzidine) substrate is introduced into the reaction, and HRP catalyzes the oxidation of TMB by H₂O₂ to produce a blue-colored product (maximum absorption peak at 655 nm). The stop solution is then added to terminate the enzymatic reaction, resulting in a yellow-colored product (maximum absorption peak at 450 nm). The absorbance at 450 nm is positively correlated with the human IFN-γ concentration in both the calibration standards and the samples. The concentration of human IFN-γ in the samples can be calculated using a dose–response curve.

Introduction

Components Shipping Condition Unit Size Detection Method
Human IFN-γ Calibration Standard 2-8℃ 96 Tests Sandwich ELISA
Anti-Human IFN-γ Microtiter Strips
Reconstitution Solution
Diluent
Wash Buffer Concentrate (10×)
Anti-hIL-6:Biotinylated Conjugate (100×)
TMB Substrate
Stop Solution
Sealing Film

 

Specification

Linearity & Range 5–400 pg/mL, R² > 0.990
Accuracy Recovery rate=88.6%–107.9%
LLOQ 5 pg/mL
ULOD 400 pg/mL
Detection limit 1 pg/mL
Specificity No cross-reactivity with human IL-2, IL-6, IL-7, IL-10, IL-12, IFNGR1, IFNGR2, IFN-α2b, IFN-β, rat IFN-γ, or mouse IFN-γ
Precision 3.9%–9.0% (CV ≤20%)
Robustness Incubation at 25 ± 3 °C, CV ≤20%
Instrument suitability not limited to MD SpectraMax ABS or Thermo Multiskan FC

 

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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