Hayflick Agar Mycoplasma Agar

Description

HAYFLICK agar is a specialized, nutrient-enriched solid medium formulated to support the growth of fastidious mycoplasmal species. The medium is supplemented with horse serum, which provides essential lipids, including cholesterol and long-chain fatty acids, required for membrane synthesis and cellular proliferation.

To fulfill nucleic acid precursor requirements, exogenous deoxyribonucleic acid (DNA) is incorporated as a source of purine and pyrimidine bases, compensating for the de novo biosynthetic deficiencies inherent to Mycoplasma spp. Metabolic activity of viable mycoplasmas leads to acid–base shifts within the medium, which are detected via the phenol red pH indicator, resulting in a visible color change from red to yellow or purple, thereby signaling active growth.

The inclusion of penicillin G suppresses the growth of background bacterial flora while maintaining selective support for mycoplasmas. Furthermore, supplementation with targeted egg-derived lipids significantly enhances colony-forming efficiency, particularly in lipid-dependent strains such as Mycoplasma pneumoniae ATCC 23714, by promoting robust membrane biogenesis and nutrient uptake.

Specifications

Application

HAYFLICK agar is recommended for the general detection of mycoplasmas in pharmaceutical products, particularly in bulk vaccines, cell banks, and virus cultures.

Features

• Ready-to-use convenience — no additional preparation steps are required.

• Broad pharmaceutical applications — suitable for biopharmaceuticals, vaccines, cell cultures, and virus cultures.

• Storage stability — can be stored at 2–8 °C for up to 6 months.

• Rapid detection — a colorimetric chart provides a quick and convenient visual indication of early-stage mycoplasma proliferation.

• Optimized growth performance — the buffered system supports higher colony counts while reducing the risk of mycoplasma cell death.

• Enhanced visualization — a chromogenic reagent selectively stains mycoplasma, improving clarity and specificity of detection under microscopy.

Documents

M-HAYFLICK-X SDS