Fungal Genomic DNA Extraction Kit

Description

Rigid chitin cell walls and heavy contamination from polysaccharides, polyphenols, or pigments often make fungal DNA extraction one of the most challenging tasks in molecular biology. Biofargo’s Fungal Genomic DNA Extraction Kit (DP317) answers these challenges with a unique, high-efficiency buffer system and silica-membrane spin columns.

This broad-spectrum kit allows you to isolate high-purity genomic DNA from tough molds, yeasts, and mushrooms in a fraction of the time—completely eliminating the need for costly Proteinase K or RNase A digestions. The recovered DNA is exceptionally stable and fully optimized for downstream applications, including PCR, restriction digestion, and next-generation sequencing (NGS) library prep.

Features

Extract DNA from Molds, yeasts & mushrooms — One Kit

Unlike standard isolation kits that only target specific fungal groups, DP317 offers a versatile, all-in-one solution across diverse sample types:  

• Molds / Filamentous Fungi: Efficiently lyses rigid species such as Aspergillus niger, Trichoderma, and Penicillium.  

• yeasts: Rapidly breaks down yeast cell walls (including Saccharomyces and Candida) without requiring tedious enzymatic pre-treatments.  

• Mushrooms / Fruiting Bodies: Easily processes dense macrofungi tissues like Lentinula edodes (Shiitake) and Flammulina velutipes (Enoki).  

• Bonus Bacteria Compatibility: Also highly effective for extracting genomic DNA from select bacteria, such as Lactobacillus and Escherichia coli.

No Proteinase K, No RNase — Faster Workflow

• Traditional protocols require hours of incubation with Proteinase K to degrade proteins, followed by secondary steps with RNase A to remove RNA.

• No Enzymes Required: DP317’s advanced lysis chemistry selectively precipitates and removes proteins, RNA, and other cellular contaminants during the initial lysis steps.

• Streamlined Protocol: Save hours on your benchwork. Go from raw sample to purified DNA without the extra incubation times or enzyme costs.

High Yield & High Purity for Inhibitor-Free Downstream Assays

Fungi are notorious for secreting secondary metabolites, polysaccharides, and pigments that co-purify with nucleic acids and shut down downstream enzymes.

Our specialized silica-column chemistry ensures that only pure genomic DNA binds to the membrane while inhibitors are completely washed away. The resulting ultra-pure DNA ensures robust reproducibility for:

• High-throughput PCR and qPCR diagnostics.

• Restriction enzyme digestion.

• Southern Blotting and hybridization assays.

• NGS Library Preparation (Illumina / Oxford Nanopore).

Specifications

Regulatory Note: This product is intended for industrial, professional, and research use only. It has not been registered as a medical device and is not for use in diagnostic or clinical medical procedures.

User-Supplied Reagents and Equipment

• Absolute ethanol

• Isopropanol

• Liquid nitrogen or a tissue homogenizer (TGrinder H24 Tissue Homogenizer, TIANGEN, SKU OSE-TH-01, recommended)

Frequently Asked Questions (FAQ)

• Does the DP317 kit really not require Proteinase K or RNase A?

  A: Correct. The kit utilizes a proprietary, uniquely formulated buffer system that lyses cell walls while simultaneously separating genomic DNA from proteins and RNA without the need for enzymatic digestion. This slashes your processing time and eliminates extra enzyme expenses.

• What specific fungal genera have been validated with this kit?

  A: This kit is highly versatile. It has been thoroughly validated on molds (including Aspergillus, Trichoderma, and Penicillium), common yeasts (Saccharomyces, Candida), and various macrofungi/mushrooms (such as Shiitake and Enoki). Additionally, it works efficiently on certain bacteria like Lactobacillus and E. coli.

• Is the eluted DNA clean enough for NGS and long-read sequencing?

  A: Yes. Because our spin column technology specifically isolates DNA while leaving polysaccharides, polyphenols, and pigments behind, the resulting genomic DNA is of high purity and integrity, making it fully compatible with library preparation for next-generation sequencing (NGS).

• How should I handle the initial sample disruption for tough fungi?

  A: Because fungal cell walls are rigid, thorough pre-treatment is essential. You can either grind the sample to a fine powder using liquid nitrogen or utilize a automated bead-beating system like the TGrinder H24 Tissue Homogenizer for maximum yield and consistency.