CHAPSO 98% Biochemical Grade

Description

CHAPSO (3‑[(3‑Cholamidopropyl)dimethylammonio]‑2‑hydroxy-1-propanesulfonate) is a high‑purity biochemical‑grade detergent supplied as a white powder, designed for membrane protein research and other sensitive biochemical applications. As a zwitterionic detergent, CHAPSO combines the ability to solubilize membrane-associated proteins with minimal disruption to their native structure and activity. Its high solubility, low UV absorbance, and mild detergent behavior make it an excellent choice for protein extraction, purification, reconstitution, and structural or functional studies.

Specifications

Features

• Gentle, non‑denaturing detergent: Preserves native conformation and biological function of membrane proteins.

• Effective membrane protein solubilization: Efficiently disrupts lipid–protein and lipid–lipid interactions for integral membrane protein extraction.

• High purity & clarity: White powder with ≥ 98% purity, yielding a clear, colorless solution with minimal interference in downstream assays.

• Low UV absorbance: Minimal background in UV-based detection, protein quantification, and structural assays.

• Dialyzable / removable by gel filtration: High critical micelle concentration and moderate micelle size allow easy removal for downstream purification or reconstitution.

Applications

• Solubilization, stabilization, and purification of integral membrane proteins while preserving native structure and function.

• Preparation of membrane protein samples for structural biology, including crystallization, cryo‑EM, NMR, and spectroscopy.

• Reconstitution of membrane proteins into lipid bilayers or liposomes for functional assays.

• Biochemical assays requiring minimal UV background interference, such as protein quantification, binding assays, and enzymatic activity measurements.

• General protein extraction and purification workflows in molecular biology and proteomics laboratories under gentle, non‑denaturing conditions.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

SDS