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For Research Use Only (RUO)
$1,010.00
Sku: 1506731
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by May 12, 2026
Product Description
The SHENTEK® BVDV Analysis Kit is an RT-qPCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of bioprocessing inputs, such as in vitro cell culture supplements and cell banks. Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), this assay ensures the efficient recovery and analysis of target nucleic acids from complex industrial matrices.
Technical Specifications
| Parameter | Details |
|---|---|
| Limit of Detection (LOD) | 50 copies per reaction |
| Assay format / reaction volume | 20 µL total reaction volume (10 µL RT-qPCR MIX + 10 µL extracted sample or control) |
| RT-qPCR MIX composition (per reaction) | BVDV Primer & Probe MIX 3.7 µL; 5×RT-qPCR Buffer 4 µL; RT-qPCR Enzyme MIX 1 µL; IPC MIX 1.3 µL; Total 10 µL |
| Thermal cycling conditions | Reverse transcription 50 °C 15:00 (1 cycle); Activation 95 °C 00:30 (1 cycle); 45 cycles of Denaturation 95 °C 00:15 and Annealing/Extension 60 °C 01:00 (fluorescence read during anneal/extension) |
| Specificity | No cross-amplification with bovine, porcine genomes, cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or expression bacteria (E. coli, Pichia pastoris) |
| Controls and acceptance criteria (Ct thresholds) | NTC: FAM Ct ≥ 40 or no amplification; NCS: FAM Ct ≥ 40 or no amplification; PCS: FAM Ct < 35 and amplification. Test sample interpretation uses FAM and VIC channels: Positive if FAM Ct < 40 (specific amplification); Presumptive positive if FAM ≥ 40 with VIC < 40 (indicates inhibitors); Negative if FAM ≥ 40 and VIC < 40 absent. |
| Kit size / throughput | Reagents for 50 reactions |
| Storage and stability | Store components at –20 °C; kit components stable up to 24 months (check label expiration date) |
| Compatible instruments | SHENTEK-96S, LightCycler 480 II, ABI 7500, Bio-Rad CFX96 and most standard real-time PCR systems supporting probe assays |
Features
- High sensitivity: Limit of detection down to 50 copies per reaction.
- High specificity: No cross-amplification observed with bovine/porcine genomes, common cell lines or typical expression organisms.
- Designed for complex matrices: Validated for detection in cell cultures and bovine serum when used with SHENTEK® Virus DNA & RNA Extraction Kit.
- Internal positive control (IPC): Includes IPC MIX to monitor for PCR inhibition and sample/extraction performance.
- Ready-to-use mixes: Pre-formulated primer/probe mix and enzyme/buffer mixes reduce setup variability.
- Platform compatibility: Compatible with multiple common real-time PCR instruments and standard probe detection settings (FAM for target, VIC for IPC).
Applications
- Qualitative molecular analysis of BVDV target sequences in industrial cell banks
- Analytical quality control of biological seed lots
- Molecular evaluation of in vitro cell culture supplements for BVDV nucleic acids
- In-process monitoring and lot-release analysis for biological manufacturing
- In vitro research projects assessing BVDV target sequences in complex culture matrices
Kit Contents
BVDV Primer & Probe MIX (NNC091)200 µL × 1 tube; –20 °C; protect from light
RT-qPCR Enzyme MIX (NNC079)100 µL × 1 tube; –20 °C
IPC MIX (NNC066)150 µL × 1 tube; –20 °C
5×RT-qPCR Buffer (NNB018)300 µL × 1 tube; –20 °C
RNase-Free H2O (NND008)1.2 mL × 1 tube; store nuclease-free (room temperature or –20 °C as per lab practice)
* Total: 50 reactions | Method: RT-qPCR
Attention
• For Research Use Only (RUO). Not for diagnostic human use.
• Read Material Safety Data Sheets (MSDS) and follow handling instructions.
• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).
• Handle positive control and extraction in a biosafety cabinet; follow BSL-2 laboratory safety guidelines.
• Use nuclease-free consumables and certified RNase/DNase-free reagents.
• Thaw kit components at 2–8 °C or on ice, vortex and spin briefly before use.
• Protect light-sensitive reagents (primer & probe mix) from light.
• If VIC (IPC) signal indicates inhibition, repeat sample preparation or remove inhibitors and retest.
• Check expiration dates on labels; store components at –20 °C.
Quality Management & Certifications
Quality System
Download QMSQMS (ISO, GMP)
Download CertificateQuality Advantages
View ReportQuality Control Process
Download ProcessTechnical Resources & Downloads
Frequently Asked Questions (FAQ)
Q1: What is the assay sensitivity (limit of detection)?
The assay sensitivity is 50 copies per reaction (LOD = 50 copies/reaction).
Q2: What samples can be tested with this kit?
The kit is intended for testing cell culture samples (≤10^7 cells), bovine serum and other sample types after appropriate extraction with the SHENTEK® Virus DNA & RNA Extraction Kit. For special sample matrices consult technical support.
Q3: What are the recommended reaction and thermal cycling conditions?
Use a 20 µL total reaction (10 µL RT-qPCR MIX + 10 µL extracted sample). Thermal program: Reverse transcription 50 °C 15:00; Activation 95 °C 00:30; 45 cycles of 95 °C 00:15 and 60 °C 01:00 (read fluorescence during annealing/extension).
Q4: What should I do if the internal control (VIC) is inhibited?
If VIC signal is inhibited, repeat sample preparation or apply inhibitor removal steps during extraction and retest the sample.
Research Use Only
✔
Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.
✔
Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
