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For Research Use Only (RUO)
$1,010.00
Sku: 1506735
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by May 12, 2026
Product Description
The SHENTEK® BPV-1 Analysis Kit is a real-time PCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of bioprocessing inputs, such as in vitro cell culture supplements and cell banks. Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), this assay ensures the efficient recovery and analysis of target nucleic acids from complex industrial matrices.
Technical Specifications
| Parameter | Details |
|---|---|
| Intended use | Qualitative detection of Bovine Parvovirus Type 1 (BPV-1) in cell banks, virus seed banks and bovine serum |
| Limit of Detection (LOD) | 50 copies per reaction |
| Specificity / Cross-reactivity | No cross-amplification detected with bovine and porcine genomes, common cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or expression bacteria/yeast (E. coli, Pichia pastoris) |
| Sample types | Cell culture (≤10^7 cells), bovine serum, and other matrices after appropriate extraction; supports complex sample matrices after extraction |
| Total reactions / Kit size | Reagents for 50 reactions |
| Reaction setup | 20 µL qPCR MIX + 10 µL template (extracted sample / control / DDB); final reaction volume 30 µL |
| qPCR mix composition (per reaction) | BPV-1 Primer&Probe MIX 2.8 µL; qPCR Reaction Buffer 15.9 µL; IPC MIX 1.3 µL; Total 20 µL |
| Thermal cycling conditions | Activation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (45 cycles); Annealing/Extension: 60 °C 01:00 (instrument reads fluorescence during this step) |
| Controls and interpretation thresholds (FAM / VIC) | NTC: Ct ≥ 40.00 or no amplification (FAM) / Ct < 35.00 for VIC indicates valid no-template control; NCS: Ct ≥ 40.00 or no amplification (FAM) / Ct < 35.00 (VIC); PCS: Ct < 35.00 (FAM and VIC); Test sample interpretation: Positive if FAM Ct < 40.00 (at least one well) with specific amplification and VIC Ct < 40.00 for duplicate runs; Presumptive positive if FAM Ct ≥ 40.00 or absent and VIC Ct < 40.00 indicating possible inhibitors, etc. See user guide tables for complete criteria. |
| Compatible instruments | SHENTEK-96S, LightCycler 480 II, ABI 7500, CFX96 and other standard real-time PCR systems supporting probe assays |
| Storage stability / Shelf life | Kit components can be stored under appropriate conditions up to 24 months (see labels) |
| Extraction compatibility | Validated for use with SHENTEK® Virus DNA & RNA Extraction Kit (Product No. 1506730) |
Features
- High sensitivity: Limit of detection down to 50 copies per reaction enables detection of low-level BPV-1 contamination.
- High specificity: No cross-amplification with bovine/porcine genomes, common mammalian cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or common expression hosts (E. coli, Pichia pastoris).
- Complete qPCR solution: Includes primer/probe mix, qPCR reaction buffer and an internal positive control (IPC) to monitor inhibition.
- Designed for complex sample matrices: Works with SHENTEK® Virus DNA & RNA Extraction Kit to recover and detect viral DNA from cells and serum.
- Broad instrument compatibility: Compatible with major real-time PCR platforms (SHENTEK-96S, LightCycler 480 II, ABI 7500, CFX96).
Applications
- Qualitative molecular analysis of BPV-1 target sequences in industrial cell banks
- Analytical quality control of biological seed lots
- Molecular evaluation of in vitro cell culture supplements for BPV-1 nucleic acids
- In-process monitoring and lot-release analysis for biological manufacturing
- In vitro research projects assessing BPV-1 target sequences in complex culture matrices
Kit Contents
BPV-1 Primer&Probe MIX (Part No. NNC095) 150 µL × 1 tube-20 °C, protect from light
qPCR Reaction Buffer (Part No. NNB001) 850 µL × 1 tube-20 °C
IPC MIX (Part No. NNC066) 150 µL × 1 tube-20 °C
DNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 1 tube-20 °C
* Total: 50 reactions | Method: qPCR
Attention
• For Research Use Only (RUO). Not for diagnostic or clinical use.
• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (protective eyewear, mask, clothing and gloves).
• Handle positive control and extraction procedures in a biosafety cabinet and follow BSL-2 laboratory safety guidelines.
• Use nuclease-free consumables and reagents certified as RNase/DNase-free.
• Thaw kit components at 2–8 °C or on ice; vortex and centrifuge briefly before use.
• Protect primer/probe mix from light.
• If VIC (internal control) signal indicates inhibition, repeat sample preparation or remove inhibitors and repeat the assay.
Quality Management & Certifications
Quality System
Download QMSQMS (ISO, GMP)
Download CertificateQuality Advantages
View ReportQuality Control Process
Download ProcessTechnical Resources & Downloads
Frequently Asked Questions (FAQ)
Q1: What is the limit of detection (LOD) for this kit?
The assay sensitivity reaches 50 copies per reaction (LOD = 50 copies/reaction).
Q2: Which sample types are supported?
The kit is validated for cell culture samples (≤10^7 cells) and bovine serum. Other sample types may be processed after appropriate extraction; consult technical support for special matrices.
Q3: What controls should be included in each run?
Include at minimum: No Template Control (NTC), Negative Control Sample (NCS), and Positive Control Sample (PCS). The guide recommends running samples in duplicate.
Q4: What should I do if the internal control (VIC) is inhibited?
If VIC signal is inhibited, repeat sample preparation or perform additional inhibitor removal steps, then re-run the assay. Persistent inhibition may require re-extraction or dilution of the nucleic acid.
Research Use Only
✔
Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.
✔
Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
