Blood SuperDirect™ PCR Kit (Heparin)-UNG

Description

This product uses a unique PCR buffer system, without DNA extraction or sample processing, it can perform direct PCR with a whole blood sample as a template. EDTA, Heparin anticoagulated whole blood and blood collection cards (such as Whatman 903® and FTA® blood collection cards) can be directly used for PCR amplification and identification, which greatly shortens the detection time. The 2× SuperEasyTM Mix provided by this kit has strong inhibitor tolerance. The maximum amount of human blood used as a PCR template can be up to 45% of the PCR system and 20% of mouse blood, which can sensitively amplify blood samples. Genomic and foreign target DNA fragments.

2× SuperEasyTM Mix-Heparin Contains our company's unique Foregene D-Taq DNA Polymerase, dNTPs, MgCl2, Taq Reaction Buffer, PCR reaction enhancer, optimizer and stabilizer.

2× SuperEasyTM Mix(UNG)-Heparin On the basis of 2×SuperEasyTM Mix-Heparin, dUTP was used instead of dTTP, and UNG enzyme (Uracil-N-glycosylase) that could degrade the dUTP template was added at the same time. Before the PCR reaction, the UNG enzyme is used to degrade the PCR product containing uracil. The UNG enzyme will not have any effect on the template that does not contain uracil, thereby ensuring the specificity and accuracy of amplification and preventing the possibility of large-scale genetic testing. Contamination of PCR products occurred.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

According to the different anticoagulants used, this product is divided into two categories: EDTA anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-EDTA) and Heparin anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-Heparin); You can also choose the corresponding kit according to the needs of the experiment, see the selection of kit type for details.

Feature

• The system has strong amplification ability and can sensitively detect the genome and foreign target DNA fragments in the blood.

• 2× SuperEasyTM Mix(UNG)-Heparin High blood tolerance: up to 45% in human blood and 20% in mouse blood.

• The sample is fully enclosed, so there is no need to worry about sample contamination and false positive PCR results. Anti-pollution PCR system 2×SuperEasyTM Mix (UNG), effectively eliminate the pollution caused by PCR products, and ensure the specificity and accuracy of amplification.

Application

Dedicated to direct PCR identification of Heparin anticoagulated whole blood. (Including: human blood, mouse blood, chicken blood, bird blood, cow blood, dog blood, etc.).

Work Flow

Storage

The whole process of low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃.

• 2× SuperEasyTM Mix(UNG)-Heparin,If used frequently, it can also be stored at 4°C for short-term storage (use up within 10 days).

• 6× DNA Loading Buffer,It can be stored at 4°C or -20°C for a long time.