Bis-Tris Propane 99% Biochemical Grade

Synonym(s): 1,3-Bis[tris(hydroxymethyl)methylamino][tris(hydroxymethyl)methylamino]propane) is a high‑quality biochemical‑grade pH buffer reagent. As a non‑zwitterionic, water‑soluble buffer, it delivers superior pH stability across a broad effective range. With dual pKₐ values (≈ 6.8 and 9.0 at 25 °C), BTP maintains stable pH in many biological and biochemical protocols. Its minimal heavy metal content and low absorbance at 260 nm/280 nm make it ideal for nucleic acid and protein-related applications.

Specifications

Features

• Wide buffering range (pH 6.3–9.5): Provides reliable pH stability across near‑neutral to mildly alkaline conditions.

• High purity (≥ 99.0%) & Biochemical Grade: Ensures minimal contaminants and low heavy‑metal content for sensitive molecular biology and diagnostic applications.

• Water‑soluble and easy to prepare: Rapidly dissolves to yield a clear, colorless solution, facilitating buffer preparation.

• Suitable for nucleic acid and protein work: Low UV absorbance at 260 nm/280 nm reduces interference in DNA/RNA quantification and protein assays.

• Versatile and stable for storage: Solid white crystalline form with a melting point around 162–165 °C; stable at room temperature when stored dry.

Applications

• Molecular biology assays: Buffer for DNA/RNA extraction, PCR, reverse transcription, and other nucleic acid manipulations.

• Protein biochemistry & enzymology: Suitable for enzyme reactions, protein purification, and assays requiring pH 6–9.5.

• Electrophoresis and chromatography: Compatible with electrophoresis buffers and chromatographic procedures requiring high purity and stable pH.

• Diagnostic / kit manufacturing: Used in biochemical diagnostic kits, nucleic acid diagnostic kits, and other assay systems.

• Protein crystallization & structural biology: Provides stable pH for crystallization trials, structural studies, and protein chemistry workflows.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

SDS