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Sku: BFEPIGMM500
Human Epithelial Cell Medium Kit
Description
Human Epithelial Cell Medium Kit is a low-serum medium system for primary epithelial cells from a wide range of tissues, including the respiratory and digestive tracts, cornea, kidney, mammary gland and prostate. It promotes attachment and spreading and maintains viability, proliferation, epithelial phenotype and key functions.
Each kit combines 480 mL of epithelial basal medium with one of two 20 mL supplements: EpiGM-M for most epithelial cell types, or EpiGM-B for retinal pigment epithelium (RPE). The supplements contain recombinant human EGF and transferrin and a small amount (2% v/v) of premium FBS to reduce senescence of primary epithelial cells.
Specifications
| Parameter | Specification |
| Product Name | Human Epithelial Cell Medium Kit |
| Catalog Numbers | BFEPIGMM500 (EpiGM-M) / BFEPIGMB500 (EpiGM-B, RPE) |
| Format | Basal medium + supplement |
| Volume | 480 mL basal medium + 20 mL supplement |
| Serum | 2% (v/v) FBS |
| Key Supplements | Recombinant human EGF, transferrin |
| pH | 7.2–7.4 |
| Endotoxin | ≤ 0.25 EU/mL |
| Filtration | 0.2 µm |
| Sterility | Bacteria and fungi not detected |
| Antibiotics | None |
| Shelf Life | 12 months |
| Intended Use | For research use only |
Features
Two matched supplements: EpiGM-M for general epithelia, EpiGM-B for retinal pigment epithelium.
Recombinant human EGF and transferrin for epithelial proliferation.
Low serum (2%) to reduce senescence of primary epithelial cells.
Supports attachment, spreading and epithelial phenotype.
Low endotoxin (≤ 0.25 EU/mL); no antibiotics or antifungals.
Applications
EpiGM-M: bronchial and alveolar, mammary, corneal and conjunctival, renal and proximal tubule, prostate epithelial cells and sebocytes.
EpiGM-B: retinal pigment epithelial (RPE) cells.
Barrier & Toxicology Studies: Epithelial monolayer and permeability assays.
Disease Modeling: Primary epithelial cultures for respiratory, renal and ocular research.
Kit Components
| Component | Volume | Storage |
| Epithelial Basal Medium | 480 mL | 4 °C |
| EpiGM-M or EpiGM-B Supplement | 20 mL | −20 °C |
Storage & Shipping
Store the basal medium at 4 °C and the supplement at −20 °C. Shelf life 12 months.
Complete medium: store at 2–8 °C and use within 4 weeks.
Shipped with ice packs and dry ice.
Protocol Overview
Thaw the supplement at 4 °C overnight or for 1–2 hours at room temperature, then add it to the basal medium (or mix 4 mL supplement with 96 mL basal medium for smaller batches).
Add 0.5–1% antibiotics if desired.
Seed primary epithelial cells at 1–1.5 × 104 cells/cm² and change the medium every other day until 70–80% confluent.
For routine passaging, seed at 5–8 × 103 cells/cm².
Frequently Asked Questions (FAQs)
Common questions about Human Epithelial Cell Medium Kit.
Which supplement should I choose?
Choose EpiGM-B for retinal pigment epithelial (RPE) cells and EpiGM-M for other epithelial cells, such as airway, mammary, corneal, renal and prostate epithelium.
Is the medium serum-free?
No. It contains a low level (2% v/v) of premium FBS to reduce senescence of primary epithelial cells.
Does it contain antibiotics?
No. Antibiotics (0.5–1%) can be added if desired.
How long is the complete medium stable?
Store at 2–8 °C and use within 4 weeks.
Can it be used for clinical or diagnostic purposes?
No. This product is for research use only and is not intended for human, diagnostic or therapeutic use.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
