Tomato Aspermy Virus (TAV) Probe Realtime RT-PCR Kit

Description

Astrovirus(AV)Probe qRT-PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

• High sensitivity: analytical LOD as low as 100 copies/reaction

• Excellent specificity: no cross-reactivity with DNA from other pathogens

• Includes amplification positive control for result verification

• Compatible with various DNA purification methods

• Suitable for both qualitative and quantitative applications

• For quantitative use: the linear range covers a 100,000-fold concentration difference.

• Sufficient for 50 reactions (20 μL each)

• For Research Use Only (RUO)

Kit Components

Application Areas

• Surveillance of Astrovirus in aquaculture (e.g., abalone, shellfish): quantitative monitoring of extracted viral nucleic acids to support stock health management — note: RNA samples must be reverse-transcribed to cDNA prior to qPCR.
• Seafood safety and quality-control testing in QC laboratories: sensitive detection and quantification of Astrovirus contamination in seafood products, processing environments, and supply-chain samples (requires appropriate nucleic acid extraction and, for RNA, reverse transcription).
• Environmental monitoring and marine ecosystem health assessment: detection and quantification of Astrovirus in seawater, sediment, and effluent samples to evaluate environmental contamination and outbreak risk (use validated extraction and reverse-transcription workflows as needed).

Storage & Shipping

• Store at - 20 °C

• The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
  

FAQ

Q: Are Biofargo PCR and qPCR Kits compatible with my thermal cycler or real-time PCR instrument?

A: Yes. Biofargo PCR Kits and probe-based qPCR Kits are designed for broad compatibility with mainstream instruments.

PCR Kits can be used with standard thermal cyclers from leading brands.

Probe-based qPCR Kits are fully compatible with major real-time PCR platforms, including ABI, Bio-Rad, Roche, QuantStudio, Qiagen Rotor-Gene Q (5-plex), and Genesig Q16.

Our technical team is available to assist with instrument setup and run optimization to ensure the best results.

Documents

Astrovirus(AV)Probe qRT-PCR Kit User Manual

MSDS