Arachidonic Acid 99%

Synonym(s): Arachidonic Acid, cis,cis,cis,cis-5,8,11,14-Eicosatetraenoic Acid, 20:4

Description

Arachidonic Acid (AA) is a naturally occurring cis-polyunsaturated omega-6 fatty acid (20:4) and a critical structural component of cell membrane phospholipids. Upon stimulation, arachidonic acid is released from membrane phospholipids by cellular phospholipases and subsequently metabolized into a wide range of bioactive eicosanoids.

These metabolites include prostaglandins and thromboxanes via the cyclooxygenase (COX) pathway, leukotrienes via the lipoxygenase (LOX) pathway, and epoxyeicosatrienoic acids (EETs) through the cytochrome P450 pathway, all of which play essential roles in inflammation, vascular tone regulation, platelet aggregation, and immune signaling.

Arachidonic acid also exhibits direct signaling activity, including covalent post-translational binding to G-protein α-subunits, inhibition of Ras-GAP, and stimulation of nitric oxide (NO) synthesis in platelets, making it a valuable reagent for biochemical, physiological, and signal transduction research.

Specifications

Biochemical & Physiological Properties

• Primary Target: Ras-GAP

• Cell Permeability: No

• ATP Competition: No

• Reversibility: No

• Mechanism of Action:

  • Precursor for prostaglandins, prostacyclin, and thromboxane

  • Modulates nitric oxide production via COX-mediated prostaglandin synthesis

  • Covalent post-translational binding to G-protein α-subunits

  • Platelet activation and NO synthesis stimulation

Features

• ≥99% purity verified by GC and TLC

• Key eicosanoid precursor for inflammatory and vascular signaling studies

• Suitable for biochemistry, pharmacology, and cell signaling research

• Packaged under inert gas to ensure chemical stability

• Consistent quality suitable for reproducible experimental results

Applications

• Prostaglandin, leukotriene, and thromboxane biosynthesis studies

• Inflammation and immune signaling research

• Platelet function and nitric oxide signaling analysis

• Lipid metabolism and membrane biology research

• Enzyme pathway studies involving COX, LOX, and cytochrome P450

Packaging & Storage

• Packaging: Packaged under inert gas

• Shipping Condition: Dry ice

• Storage Temperature: −70 °C

• Storage Instructions:

  • OK to freeze

  • Protect from light

Preparation & Stability

• Following reconstitution, aliquot and store at −20 °C

• Stock solutions are stable for up to 1 month at −20 °C

• Avoid repeated freeze–thaw cycles to maintain integrity

Safety & Disclaimer

• Toxicity: Harmful (C)

• For Research Use Only (RUO)

• Not for diagnostic or therapeutic use