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$160.00
Sku: GMP-TL101-0500
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by November 13, 2026
Description
Anti-CD3 stimulation is the starting point of most T cell activation and expansion workflows, including CAR-T, TIL and CIK cell production. For these workflows the antibody itself becomes part of the process, so endotoxin, host cell residues, sterility and lot consistency matter as much as binding.
This anti-human CD3 monoclonal antibody is a recombinant humanized, full-length IgG1 that binds the extracellular domain of the CD3ε chain. Crosslinking the TCR–CD3 complex delivers the primary activation signal to T cells; it is typically combined with CD28 costimulation and IL-2 or other cytokines to drive expansion.
The antibody is expressed in CHO cells grown in animal component-free media, purified by Protein A affinity chromatography and formulated in phosphate-buffered saline with human serum albumin. It contains no BSA and no sodium azide. Each lot is released against specifications for purity, endotoxin (<0.01 EU/µg), sterility, mycoplasma, CHO host cell protein and DNA, and Jurkat cell binding.
Specifications
| Cat. No. | GMP-TL101-0500 |
| Size | 500 µg |
| Target | Human CD3ε (extracellular domain) |
| Format | Recombinant humanized monoclonal antibody, full-length IgG1 |
| Expression System | CHO cells, animal component-free media |
| Purification | Protein A affinity chromatography |
| Form | Liquid, sterile |
| Concentration | 1.0–1.5 mg/mL (see lot COA) |
| Formulation | 20 mM phosphate buffer, 150 mM NaCl, pH 7.4, with human serum albumin. No BSA, no sodium azide. |
| Storage | 2–8°C, under sterile conditions. Do not freeze. |
| Shelf Life | 24 months at 2–8°C |
| Grade | GMP-grade cell and gene therapy (CGT) reagent. For research and manufacturing use; not for direct human administration or diagnostic use. |
Quality Control Specifications
| Test | Specification |
|---|---|
| Appearance | Colorless, transparent liquid; no visible particles |
| Identity | SDS-PAGE bands consistent with product molecular weight |
| pH | 6.5–7.5 |
| Concentration | 1.0–1.5 mg/mL |
| Purity (SDS-PAGE, HPLC) | >95% |
| Endotoxin (LAL) | <0.01 EU/µg |
| Sterility | No growth |
| Mycoplasma | Negative |
| CHO Host Cell Protein | ≤100 ppm |
| CHO Host Cell DNA | ≤50 ng/mg |
| Biological Activity | Binding rate to Jurkat cells >90% |
Lot-specific results are reported on the Certificate of Analysis. Example lot mAb2406001: concentration 1.04 mg/mL, purity 98.8%, CHO-HCP 1.23 ppm, CHO DNA not detected, Jurkat binding 97.3%.
Features
- Recombinant humanized IgG1 — consistent, sequence-defined antibody produced in CHO cells.
- Process-ready formulation — PBS with human serum albumin; free of BSA and sodium azide, so it can go straight into cell culture.
- Released with CGT-relevant QC — sterility, mycoplasma, endotoxin <0.01 EU/µg, CHO HCP and DNA residues.
- Function-tested — every lot tested for binding to CD3+ Jurkat cells (>90%).
- Long shelf life — 24 months at 2–8°C.
Applications
- T cell activation and expansion — primary (signal 1) stimulation of human PBMC-derived T cells, used plate-bound or in solution with CD28 costimulation and cytokines.
- CAR-T and TCR-T process development — T cell activation prior to transduction and during ex vivo expansion.
- TIL and CIK cell culture — stimulation step in tumor-infiltrating lymphocyte and cytokine-induced killer cell protocols.
- T cell functional assays — PBMC stimulation for cytokine release, proliferation and activation marker readouts.
Usage Guidance
Optimal concentration depends on cell source, culture vessel and whether costimulation is used, and should be determined by titration. As a general starting point for anti-CD3 stimulation:
- Plate-bound: coat wells or flasks with 1–5 µg/mL in sterile PBS for 2 hours at 37°C or overnight at 2–8°C, then rinse with PBS before adding cells.
- Soluble: add directly to culture medium, typically in the ng/mL range, with a CD28 signal and cytokines such as IL-2.
These are general reference ranges for anti-CD3 stimulation, not lot-specific validated conditions. Handle aseptically; the product is supplied sterile and contains no preservative other than human serum albumin.
Documents
FAQ
Is this antibody mouse or humanized?
It is a recombinant humanized full-length IgG1 expressed in CHO cells.
Does it contain sodium azide or BSA?
No. It is formulated in 20 mM phosphate buffer with 150 mM NaCl, pH 7.4, and human serum albumin.
What is it mainly used for?
Activation and expansion of human T cells, including CAR-T, TIL and CIK workflows. It is not positioned as a detection antibody for Western blot or immunofluorescence.
What concentration should I use?
Titrate for your system. A common starting point is 1–5 µg/mL for plate coating; soluble use is typically in the ng/mL range with CD28 costimulation.
Is it GMP-grade?
It is supplied as a GMP-grade CGT reagent for research and manufacturing use. It is not intended for direct administration to humans or for diagnostic use.
How should it be stored?
Store at 2–8°C under sterile conditions; do not freeze. Shelf life is 24 months.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
