For Research Use Only (RUO)

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Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by October 03, 2026
Detection Method
ELISA
Assay Time
Refer to manual
Storage Temperature
2-8 °C (short-term); < -65 °C (stable for 12 months)

Product Description

SHENTEK™ Anti-E.coli (K-12 & Alkaline Lysis) HCP Antibody is an affinity-purified polyclonal sheep IgG raised against Escherichia coli host cell proteins (HCPs) derived from K-12 strains prepared by alkaline lysis. The reagent is supplied as a liquid, unconjugated antibody in PBS without preservatives and is intended for research applications including HCP coverage evaluations, Western blot and ELISA. Supplied at 1 mg per vial. Store at 2-8 °C for short-term use and below -65 °C for long-term storage. This product is for research use only (RUO) and is not for diagnostic purposes.

Technical Specifications

Parameter Details
Product No. 1301302-Ab01
Size 1 mg
Species Reactivity Escherichia coli (E. coli), K-12 strains
Host / Isotype Sheep / IgG
Class Polyclonal
Type Antibody, unconjugated
Immunogen E. coli HCPs (K-12 strains) produced chemically by alkaline lysis
Form Liquid
Purification Affinity Purified
Storage buffer PBS
Contains No preservatives
Applications HCP Coverage evaluations, Western Blot, ELISA
Storage Maintain at 2-8 °C for short-term use. For long-term storage, store below -65 °C.
Validity period 12 months (when stored as recommended)

Features

  • Affinity-purified polyclonal antibody: Provides broad coverage of E. coli host cell proteins derived from K-12 strains.
  • Produced against alkaline-lysis HCPs: Immunogen produced by chemical alkaline lysis to represent HCPs from K-12 strains.
  • Unconjugated format: Suitable for use as primary antibody in ELISA, Western blot and HCP coverage evaluation workflows.
  • No preservatives: Formulated in PBS without preservatives to minimize interference in downstream assays.

Applications

  • HCP coverage evaluations
  • Western Blot (WB)
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Research use in process development and HCP monitoring for E. coli-derived products

Kit Contents

Anti-E.coli (K-12 & Alkaline Lysis) HCP Antibody, 1 mg2-8 °C (short-term); < -65 °C (long-term)
Storage buffer: PBS2-8 °C
* Total: N/A (antibody reagent) | Method: ELISA / Western Blot (antibody compatibility)

Attention

• For research use only. Not intended for diagnostic purposes.

• Maintain at 2-8 °C for short-term use; for long-term storage store below -65 °C.

• Do not freeze-thaw repeatedly; aliquot to avoid multiple freeze-thaw cycles.

• Contains no preservatives; handle under aseptic conditions where appropriate.

Quality Management & Certifications

Quality System

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QMS (ISO, GMP)

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Quality Advantages

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Quality Control Process

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📂 Technical Resources & Downloads

Frequently Asked Questions (FAQ)

Q1: What is the recommended storage condition for this antibody?
Store at 2-8 °C for short-term use. For long-term storage, keep below -65 °C. Product is stable for 12 months under recommended storage.
Q2: What applications is this antibody validated for?
This antibody is intended for HCP coverage evaluations and is suitable for use in Western blot and ELISA-based assays.
Q3: Is this product suitable for diagnostic use?
No. This product is provided for research use only (RUO) and is not intended for diagnostic purposes.
Q4: What is the immunogen used to generate this antibody?
The antibody was raised against E. coli host cell proteins (K-12 strains) produced chemically by alkaline lysis.

Research Use Only

✔

Research Use Only (RUO) - Not for diagnostic use – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.

✔

Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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