2X Taq PCR Master Mix (with Dye)

Background

The 2X Taq PCR Master Mix (with dye) is a ready-to-use reagent designed for DNA amplification by polymerase chain reaction (PCR). It contains recombinant Taq DNA polymerase derived from Thermus aquaticus and produced in an E. coli expression system. Taq polymerase catalyzes DNA synthesis by incorporating nucleotides onto primer-template complexes. This polymerase exhibits 5'→3' polymerase activity along with weak 5'→3' exonuclease activity and lacks proofreading (3'→5' exonuclease) functionality, leaving adenine overhangs at amplified fragment 3' ends, facilitating subsequent TA cloning assays. The added dye permits direct loading of PCR products onto agarose gels without additional loading buffers. This reagent is suitable for routine molecular biology applications including genotyping, cloning, and DNA sequence analysis, assisting researchers to reduce experimental variability by minimizing handling steps.

Specification

Features

• Ready-to-Use 2X Formulation

  Pre-mixed with Taq DNA polymerase, MgCl₂, dNTPs, PCR buffer, and enhancers for fast PCR setup.

• Direct Gel Loading

  Contains bromophenol blue dye, enabling PCR products to be loaded directly onto agarose gels.

• Reliable Amplification Performance

  Optimized buffer system supports stable and efficient amplification in routine PCR assays.

• TA Cloning Compatible

  Generates PCR products with 3′-dA overhangs, facilitating efficient ligation into T-vectors.

• Enhanced Stability

  Formulated with stabilizers; can be stored at 4 °C for up to 3 months and long-term at −20 °C.

Applications

• Conventional PCR amplification

• Gene cloning and insert preparation

• Genotyping and mutation analysis

• Template preparation for DNA sequencing

• Routine molecular biology research workflows

Storage

Store the 2X master mix at -20°C.

Documents

2X Taq PCR Master Mix (with Dye)