15% One-Step PAGE Color Gel Quick Preparation Kit

Description

Traditional SDS-PAGE gel preparation requires multiple manual procedures, including precise acrylamide concentration adjustment, separate preparation of resolving and stacking gels, polymerization reagent handling, and repeated optimization for different protein sample types. These steps can increase preparation time and introduce variation, especially when high-resolution separation of small proteins or peptides is required.

The 15% One-Step SDS-PAGE Color Gel Fast Preparation Kit (RP0261) is a complete SDS-PAGE gel preparation kit designed for high-resolution separation of low molecular weight proteins and peptide samples using polyacrylamide gel electrophoresis. The kit provides pre-optimized separation gel solution, concentrating gel solution, corresponding buffers, and a novel coagulant system, allowing researchers to prepare PAGE gels without additional TEMED addition or complex reagent calculations.

Designed for the Tris-glycine SDS-PAGE system, the RP0261 One-Step PAGE Gel Kit contains a 15% polyacrylamide gel formulation with smaller pore sizes for improved resolution of small proteins compared with lower percentage SDS-PAGE gels. It is particularly suitable for applications requiring enhanced separation of proteins below approximately 50 kDa, including detailed protein characterization and high-resolution Western blot analysis.

The colored concentrating gel included in this protein gel casting kit improves visualization of sample loading wells and supports accurate sample application during electrophoresis. Compared with conventional hand-cast SDS-PAGE methods, the TEMED-free SDS-PAGE gel preparation workflow reduces preparation complexity and improves consistency for laboratories performing routine and advanced protein analysis.

The RP0261 kit is intended for research use only and is suitable for applications including small protein separation, peptide electrophoresis, Western blot gel preparation, protein expression analysis, and polyacrylamide gel electrophoresis.

Specification

Kit Components

Features

• Optimized 15% SDS-PAGE gel formulation: Provides a high-percentage polyacrylamide matrix with smaller pores for improved separation of low molecular weight proteins.

• High-resolution protein separation: The 15% SDS-PAGE gel improves band resolution for small proteins and peptide samples compared with lower percentage gels.

• One-step SDS-PAGE gel preparation: Pre-formulated solutions simplify gel casting by reducing manual calculations and preparation procedures.

• TEMED-free gel preparation: The optimized coagulant system simplifies polymerization without requiring additional TEMED addition during gel preparation.

• Rapid PAGE gel preparation: Complete gel polymerization can be achieved in approximately 15 minutes, improving laboratory workflow efficiency.

• Consistent protein resolution: The pre-optimized formulation helps reduce variability associated with conventional hand-cast SDS-PAGE gels.

Applications

The 15% One-Step SDS-PAGE Color Gel Fast Preparation Kit is designed for laboratories requiring high-resolution separation of small proteins and peptide samples using polyacrylamide gel electrophoresis.

• Small protein separation: Suitable for resolving low molecular weight proteins that require higher percentage SDS-PAGE gels for improved separation.

• Peptide electrophoresis: Provides enhanced resolution for peptide samples and very small protein fragments.

• Western blot analysis: Preparation of SDS-PAGE gels for detection of low molecular weight proteins before membrane transfer.

• Protein characterization: Analysis of purified proteins, protein fragments, and molecular weight differences.

• Protein expression analysis: Evaluation of recombinant proteins and engineered protein products with lower molecular weights.

• Biotechnology and academic research: Convenient high-resolution SDS-PAGE gel preparation for molecular biology laboratories.

Choosing the Right SDS-PAGE Gel Percentage

The optimal SDS-PAGE gel concentration depends on protein molecular weight and the required separation resolution. Lower percentage polyacrylamide gels provide larger pores for high molecular weight proteins, while higher percentage gels provide improved resolution for smaller proteins. The 15% SDS-PAGE gel contains a higher acrylamide concentration and smaller pore structure, making it suitable for high-resolution separation of small proteins, protein fragments, and peptide samples.

Preparation Workflow

The RP0261 One-Step SDS-PAGE Gel Preparation Kit provides a simplified workflow for preparing high-resolution protein electrophoresis gels. The pre-optimized formulation reduces manual preparation steps while maintaining compatibility with standard Tris-glycine SDS-PAGE systems.

1. Mix equal volumes of Separation Gel Solution and Separation Gel Buffer to prepare the resolving gel mixture.

2. Add Novel Coagulant to the separation gel solution and gently mix to initiate polymerization while avoiding bubble formation.

3. Transfer the prepared resolving gel solution into the gel cassette.

4. Mix Concentrating Gel Solution and Concentrating Gel Buffer to prepare the stacking gel solution.

5. Add Novel Coagulant and directly cast the concentrating gel above the separation gel layer without waiting for complete resolving gel polymerization.

6. Insert the comb and allow approximately 15 minutes for gel polymerization before performing SDS-PAGE electrophoresis.

The simplified casting procedure reduces preparation complexity and improves reproducibility for laboratories performing high-resolution separation of small proteins and peptide samples.

Technical Notes

• The RP0261 kit is designed for denaturing SDS-PAGE electrophoresis. Separation and concentrating gel buffers contain SDS and are not intended for native PAGE applications.

• The 15% polyacrylamide formulation provides smaller pore sizes than lower percentage SDS-PAGE gels, making it suitable for enhanced resolution of low molecular weight proteins.

• The colored concentrating gel improves visualization of sample loading wells and supports accurate sample application during electrophoresis.

• High-percentage SDS-PAGE gels may provide improved separation of small proteins but may require optimization of electrophoresis conditions depending on sample properties.

• Gel polymerization speed is temperature dependent. Lower temperatures may extend polymerization time, while suitable room temperature conditions support efficient gel formation.

• For optimal electrophoresis performance, freshly prepared gels are recommended for use.

Documents

Manual

MSDS

FAQ