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$98.40
Sku: RP0241
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by July 15, 2026
Description
Traditional SDS-PAGE gel preparation requires multiple manual steps, including gel concentration calculations, separate preparation of resolving and stacking gels, polymerization reagent handling, and optimization of casting conditions. These additional procedures may increase experimental preparation time and introduce variation that affects protein separation consistency, especially in routine Western blot and protein expression analysis workflows.
The 10% One-Step SDS-PAGE Color Gel Fast Preparation Kit (RP0241) is a complete SDS-PAGE gel preparation kit designed for routine protein electrophoresis applications. The kit provides pre-optimized separation gel solution, concentrating gel solution, corresponding buffers, and a novel coagulant system to simplify polyacrylamide gel preparation without additional TEMED addition or complicated reagent calculations.
Designed for the Tris-glycine SDS-PAGE system, the RP0241 One-Step PAGE Gel Kit provides a balanced 10% polyacrylamide gel formulation suitable for general protein separation and commonly used Western blot gel preparation workflows. The 10% SDS-PAGE gel concentration offers a versatile pore size for resolving a broad range of commonly analyzed proteins, making it a preferred choice for routine protein expression analysis and molecular biology applications.
The colored concentrating gel included in this protein gel casting kit improves visualization of sample wells and supports accurate loading during electrophoresis. By integrating optimized gel preparation reagents with a TEMED-free SDS-PAGE gel preparation workflow, RP0241 helps laboratories reduce preparation complexity while improving consistency between experiments.
The RP0241 kit is intended for research use only and is suitable for applications including Western blot analysis, protein expression detection, recombinant protein characterization, and polyacrylamide gel electrophoresis.
Specification
| Product Name | 10% One-Step SDS-PAGE Color Gel Fast Preparation Kit |
| SKU | RP0241 |
| Gel Type | Polyacrylamide SDS-PAGE Gel |
| Gel Concentration | 10% |
| Electrophoresis System | Tris-Glycine SDS-PAGE System |
| Recommended Protein Range | Approximately 20–150 kDa proteins |
| Application | Protein electrophoresis, Western blotting, protein expression analysis |
| Gel Preparation Method | One-step gel casting workflow |
| Polymerization Time | Approximately 15 minutes |
| Storage Condition | 4 °C; Novel Coagulant long-term storage at −20 °C |
| Shelf Life | 18 months |
| Research Use | For Research Use Only (RUO) |
Kit Components
| Component | Volume |
| Concentrating Gel Solution | 80 mL |
| Concentrating Gel Buffer | 80 mL |
| Separation Gel Solution | 250 mL |
| Separation Gel Buffer | 250 mL |
| Novel Coagulant | 8 mL |
Features
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Optimized 10% SDS-PAGE gel formulation: Provides a balanced pore structure suitable for routine protein separation and commonly analyzed molecular weight ranges.
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Ideal for routine Western blot workflows: The 10% gel concentration offers versatile separation performance for frequently studied proteins in molecular biology laboratories.
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One-step SDS-PAGE gel preparation: Pre-formulated gel solutions simplify polyacrylamide gel casting by reducing manual preparation steps and concentration calculations.
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TEMED-free PAGE gel preparation: The optimized coagulant system eliminates the need for additional TEMED addition during gel preparation.
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Rapid SDS-PAGE gel preparation: Complete gel polymerization can be achieved in approximately 15 minutes, improving laboratory workflow efficiency.
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Consistent protein separation: Pre-optimized reagents help reduce variability associated with conventional hand-cast SDS-PAGE gel preparation.
Applications
The 10% One-Step SDS-PAGE Color Gel Fast Preparation Kit is designed for laboratories requiring reliable and versatile protein separation performance for routine polyacrylamide gel electrophoresis workflows.
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Western blot analysis: Preparation of SDS-PAGE gels for routine protein transfer and antibody-based detection experiments.
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Routine protein expression analysis: Evaluation of protein expression levels from bacterial, mammalian, and other biological expression systems.
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Recombinant protein characterization: Analysis of purified recombinant proteins and protein expression products.
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Protein molecular weight analysis: Determination of relative protein size and comparison of protein expression profiles.
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Cell lysate protein analysis: Separation of proteins from biological samples for downstream detection and characterization.
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Biotechnology and academic research: A convenient SDS-PAGE gel preparation solution for routine laboratory protein analysis workflows.
Choosing the Right SDS-PAGE Gel Percentage
The appropriate SDS-PAGE gel concentration depends on protein molecular weight, sample complexity, and the desired separation resolution. Lower percentage gels provide larger pores for high molecular weight proteins, while higher percentage gels improve resolution for smaller proteins. The 10% SDS-PAGE gel is widely used as a general-purpose protein separation gel because it provides balanced migration and resolution across many routine Western blot and protein analysis applications.
| Gel Concentration | Recommended Application | Typical Protein Range |
| 6% SDS-PAGE Gel | High molecular weight proteins and large protein complexes | >100 kDa proteins |
| 8% SDS-PAGE Gel | Medium to high molecular weight protein separation | Approximately 50–200 kDa proteins |
| 10% SDS-PAGE Gel | General protein electrophoresis and routine Western blot workflows | Approximately 20–150 kDa proteins |
| 12% SDS-PAGE Gel | Routine protein separation with improved resolution for smaller proteins | Approximately 10–100 kDa proteins |
| 15% SDS-PAGE Gel | Small protein, peptide, and low molecular weight protein analysis | <50 kDa proteins |
Preparation Workflow
The RP0241 One-Step SDS-PAGE Gel Preparation Kit provides a simplified protein gel casting workflow compared with conventional SDS-PAGE preparation methods. Pre-optimized solutions reduce manual preparation steps while maintaining compatibility with standard Tris-glycine electrophoresis systems.
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Mix equal volumes of Separation Gel Solution and Separation Gel Buffer to prepare the resolving gel mixture.
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Add Novel Coagulant to the separation gel mixture and gently mix to initiate polymerization while avoiding bubble formation.
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Transfer the prepared separation gel solution into the gel cassette.
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Mix Concentrating Gel Solution and Concentrating Gel Buffer in equal volumes to prepare the stacking gel solution.
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Add Novel Coagulant to the concentrating gel mixture and directly cast the stacking gel above the separation gel layer without waiting for complete resolving gel polymerization.
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Insert the comb and allow approximately 15 minutes for gel polymerization before starting SDS-PAGE electrophoresis.
The simplified casting procedure improves workflow efficiency for routine protein electrophoresis experiments while reducing variability associated with traditional manual PAGE gel preparation.
Technical Notes
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The RP0241 kit is designed for denaturing SDS-PAGE electrophoresis. Separation and concentrating gel buffers contain SDS and are not intended for native PAGE applications.
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The 10% polyacrylamide gel formulation provides a balanced separation range suitable for many commonly analyzed proteins in Western blot and protein expression studies.
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The colored concentrating gel helps researchers identify loading wells during sample application and improves loading consistency.
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Gel polymerization speed is affected by temperature. Higher temperatures may accelerate polymerization, while lower temperatures may require additional polymerization time.
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For optimal protein separation performance, freshly prepared SDS-PAGE gels are recommended for electrophoresis experiments.
Documents
FAQ
Why is a 10% SDS-PAGE gel commonly used for protein analysis?
A 10% SDS-PAGE gel provides a balanced pore size that allows effective separation of many commonly studied proteins. It is widely used for routine Western blotting, protein expression analysis, and general protein electrophoresis because it covers a broad molecular weight range.
What proteins are suitable for RP0241 10% SDS-PAGE gel?
The RP0241 10% SDS-PAGE gel is suitable for proteins in the approximate range of 20–150 kDa, depending on electrophoresis conditions and sample characteristics. It is commonly selected for routine protein separation workflows.
Can RP0241 be used for Western blot gel preparation?
Yes. RP0241 is designed for SDS-PAGE protein separation before membrane transfer and antibody detection. The 10% gel concentration provides versatile separation performance for many Western blot applications.
What are the advantages of a one-step SDS-PAGE gel preparation kit?
A one-step SDS-PAGE gel preparation kit simplifies traditional gel casting by providing pre-optimized solutions and reducing the need for multiple preparation steps. This helps improve laboratory efficiency and reproducibility.
Does RP0241 require additional TEMED during gel preparation?
No. RP0241 uses an optimized coagulant system and does not require additional TEMED addition during the gel preparation process, simplifying SDS-PAGE gel casting.
Is RP0241 compatible with standard SDS-PAGE electrophoresis equipment?
Yes. RP0241 is designed for standard Tris-glycine SDS-PAGE systems and can be used with conventional PAGE gel casting and electrophoresis equipment.
What is the difference between RP0241 10% gel and RP0231 8% gel?
The RP0231 8% SDS-PAGE gel is optimized for medium to high molecular weight proteins, while RP0241 10% SDS-PAGE gel provides a more balanced separation range for routine protein analysis and Western blot workflows.
Can RP0241 replace traditional hand-cast SDS-PAGE gels?
RP0241 provides a simplified alternative to conventional hand-cast SDS-PAGE gel preparation by combining optimized gel solutions with a rapid polymerization workflow while maintaining compatibility with standard protein electrophoresis applications.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
