Tomato Endogenous Apx Gene Probe Realtime PCR Kit

Description

Tomato Endogenous Apx Gene Probe Realtime PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

• High sensitivity: analytical LOD as low as 100 copies/reaction

• Excellent specificity: no cross-reactivity with DNA from other pathogens

• Includes amplification positive control for result verification

• Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.

• Suitable for both qualitative and quantitative applications

• For quantitative use: the linear range covers a 100,000-fold concentration difference.

• Sufficient for 50 reactions (20 μL each)

• For Research Use Only (RUO)

Kit Components

Application Areas

• Seed Certification & Breeding Optimization: Rapid quantification of the endogenous Apx gene for high‐throughput screening of tomato germplasm, enabling precision selection in stress‐tolerance breeding programs and ensuring genetic purity in seed production.
• GMO Compliance & Regulatory Testing: Sensitive detection of native Apx targets to discriminate non‐modified lines from transgenic events, supporting regulatory submissions, import/export inspections and on‐site audit requirements under global GMO legislation.
• Food Safety & Product Traceability: Authentication and quality control of tomato‐based ingredients in processed foods, sauces and juices by verifying endogenous Apx content to prevent adulteration, ensure label accuracy and meet consumer‐driven traceability standards.

Storage & Shipping

• Store at - 20 °C

• The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
  

Documents

Tomato Endogenous Apx Gene Probe Realtime PCR Kit User Manual

MSDS