Description

This kits intended for quantitative determination of kanamycin residues in plasmid DNA, that is used in the development of cell and gene therapies.

The SHENTEK^® Kanamycin ELISA Kit utilizes an "indirect-competitive" enzyme immunoassay. The microplate wells are pre-coated with kanamycin antigen, which competes with kanamycin residues in the sample for binding to the anti-kanamycin monoclonal antibody. An enzyme-labeled secondary antibody then targets the primary monoclonal antibody that has formed complexes with the kanamycin antigen coated on the plate wells. Upon addition of the TMB substrate, the resulting color intensity measured at 450 nm exhibits an inverse relationship with the concentration of kanamycin residues in the sample. The total amount of kanamycin in the sample is obtained by comparison with a standard curve, and adjusted by the sample dilution factor.

For the detection of residual kanamycin in other biologics samples, sample suitability test is recommended to assess the effects of matrix interference.

Components	Shipping Condition	Unit Size	Detection Method
Kanamycin Standard	2-8℃, protect from light	96 Tests	Sandwich ELISA
Kanamycin Standard Reconstitution Diluent	2-8℃
Kanamycin Standard Solution Diluent	2-8℃
100×Kanamycin Primary Antibody	2-8℃
100×Enzyme-conjugated Secondary Antibody	2-8℃, protect from light
Kanamycin-coated Plate	2-8℃, protect from light
10×Wash Buffer	2-8℃
Chromogenic Substrate for Kanamycin ELISA	2-8℃, protect from light
Stop Solution	2-8℃
Sealing Film	2-8℃

Specifications

Linearity & Range	0.5-32 ng/mL, R2>0.990
Accuracy	Recovery Rate= 80.8%-112.5%
LLOQ	0.5 ng/mL
ULOQ	16 ng/mL
Repeatability	5.3%-9.1% (CV≤20%)
Specificity	No cross-reactivity with tetracycline, streptomycin sulfate, ampicillin, and chloramphenicol
Robustness	Incubate at 22℃ to 30℃, CV≤ 20%
Robustness	Instrument suitability not limited to Thermo Multiskan FC, Bio-Tek Synergy2

Related Products

SHENTEK^® Residual Host Cell DNA Sample Preparation Kit

Documents

User Guide

Research Use Only

✔

For Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.

✔

Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.

Kanamycin ELISA Kit SHENTEK®