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| Protein sequence | A DNA sequence encoding the human Fibronectin (P02751) was expressed with a His-tag at the N-terminus |
| Expression Host | E. coli |
| QC Testing Purity | > 90 % as determined by SDS-PAGE |
| Activity | By measuring the adhesion capacity of NIH-3T3 mouse embryonic fibroblasts. When cells are added to the FN coated well plate (2.5μg/mL and 100μL/well), about 35%~60% of the cells will be specifically attached after 35 minutes at 37℃ |
| Endotoxin Level | < 0.1 EU per 1 μg of the protein as determined by the LAL method |
| Molecular Mass | The Recombinant Human Fibronectin Protein predicts a molecular mass of 64.7 kD |
| Formulation | Supplied as a 0.22μm filtered solution in PBS, PH 7.4 |
| Stability & Storage | 24 months at 2 °C to 8 °C. Avoid repeated freeze-thaw cycles. |
Background
Recombinant Human Fibronectin (FN) Protein , including three functional regions (cell binding domain, heparin binding domain, and CS1 site), can significantly enhance retrovirus mediated gene transfer to mammalian cells. This effect may be due to colocalization of retroviral particles and target cells on rhFN CH-296 chimeric molecule via retroviral particles or heparin binding region II binding to cell adhesion sites VLA-4 and/or VLA-5. According to the research results of Hannenberg et al., there is typically 50% to 75% gene transduction of cells with the coating method.
References
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Williams CM, Engler AJ, Slone RD, Galante LL, Schwarzbauer JE ( 2008) Fibronectin expression modulates mammary epithelial cell proliferation during acinar differentiation. Cancer Research 68 (9):3185–3192. 2. Mao Y, Schwarzbauer JE (2005) Fibronectin fibrillogenesis, a cell-mediated matrix assembly process. Matrix Biology : Journal of the International Society for Matrix Biology 24 (6): 389–399. 3. Erickson HP (2002) Stretching fibronectin. Journal of Muscle Research and Cell Motility 23 (5-6):575–580.
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