Specification

Full name	ZYX rabbit monoclonal antibody
Alternative names	50 μl/100 μl
Reactivity	rabbit monoclonal
Applications	WB, IHC, IF
Host	Rabbit
Clone type	rabbit monoclonal
Target Background	Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008]
Swissprot No	Q15942
Gene Accession	7791
WB Predicted band size	61 kDa
WB Positive control
WB Recommended dilution	500-2000
IHC predicted cell location	Predicted cell location: Cytoplasm
IHC positive control	Positive control: Human lung cancer
IHC Recommed dilution	Recommended dilution: 50-100

Storage

-20°C, 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA. Avoid repeated freeze-thaw cycles.

P291686-WB-1-P

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using P291686(ZYX Antibody) at dilution 1/50