Description

Yeast Nitrogen Base without Amino Acids contains all essential nutrients and vitamins and inorganic salts necessary for the cultivation of yeasts except histidine, methionine, tryptophane and a source of carbohydrate.

Formula (per Liter)

Ammonium Sulfate	5.0g
Biotin	0.002mg
Folic Acid	0.002mg
Niacin	0.4mg
Pyridoxine Hyrochloride	0.4mg
Thiamine Hydrochloride	0.4mg
Copper Sulfate	0.04mg
Ferric Chloride	0.2mg
Sodium Molybdate	0.2mg
Monopotassium Phosphate	1.0g
Sodium Chloride	0.1g
Calcium Pantothenate	0.4mg
Inositol	2mg
p-Aminobenzoic Acid	0.2mg
Riboflavin	0.2mg
Boric Acid	0.5mg
Potassium Iodide	0.1mg
Manganese Sulfate	0.4mg
Zinc Sulfate	0.4mg
Magnesium Sulfate	0.5g
Calcium Chloride	0.1g

Final pH: 5.4 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance

Use

Yeast Nitrogen Base without Amino Acids is used for classifying yeasts based on
amino acid and carbohydrate requirements.

Preparation

Prepare a 10X solution by dissolving 6.7 grams of Yeast Nitrogen Base without Amino Acids, 5 grams of Dextrose or equivalent amount of other carbohydrate and 5-10 mg of the desired amino acid in 100ml distilled or deionized water. Warm slightly to dissolve. Mix well. DO NOT BRING TO A BOIL. DO NOT AUTOCLAVE. Filter-sterilize the solution. Store at 2-8°C. Prepare the final medium by aseptically pipetting 0.5ml of the 10X solution into 4.5ml of sterile distilled water. Mix the solution thoroughly by shaking before inoculation.

Quality Control Specifications

The powder is homogeneous, free flowing and off-white.
Visually the prepared medium is clear and almost colorless.
Expected cultural response after 2-5 days at 25.0°C.

Organism	Result with Additives
Candida albicans ATCC 10231	Good Growth
Kloeckera apiculata ATCC 9774	Good Growth
Saccharomyces pastorianus ATCC 9080	Good Growth

Storage

Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original off-white color.

Documents

INFO

SDS

Yeast Nitrogen Base w/o Amino Acids (S507)