Name: XR Epithelial Cell Growth Medium Kit
Brand: XR
SKU: XREpiGM-500-A

Does the medium contain antibiotics?

No. The supplied medium does not contain antibiotics or antifungal agents. An antibiotic solution may be added at 0.5–1% when required by the laboratory protocol.

How should the complete epithelial cell medium be prepared?

Thaw the appropriate EpiGM supplement at 4°C overnight or at room temperature for 1–2 hours, then mix it completely with the Epi basal medium. For partial preparation, combine 4 mL of supplement with 96 mL of basal medium.

Description

Primary epithelial cells can be difficult to establish and expand because limited attachment, slow spreading, early senescence and loss of epithelial characteristics may reduce experimental consistency. A general-purpose basal medium may not provide the tissue-adapted nutrients and growth support required by sensitive primary epithelial cell cultures.

XR Epithelial Cell Growth Medium Kit is a low-serum primary epithelial cell culture medium developed for epithelial cells derived from respiratory, ocular, renal, mammary, prostate and other tissues. Its optimized basal formulation supports cell attachment and spreading, while the selected epithelial growth supplement provides balanced nutritional support for cell survival and proliferation.

The complete culture system contains recombinant human epidermal growth factor and transferrin together with 2% v/v fetal bovine serum. This low-serum formulation is designed to reduce excessive serum exposure while providing the growth support needed to help delay primary epithelial cell senescence and maintain epithelial phenotype and key cellular functions.

Two tissue-oriented supplement options are available. EpiGM-B is intended for retinal pigment epithelial cell culture, while EpiGM-M supports a broader range of primary epithelial cell types, including bronchial, alveolar, mammary, corneal, conjunctival, renal, renal proximal tubule, sebaceous gland and prostate epithelial cells.

Important: XR Epithelial Cell Growth Medium is a low-serum medium containing 2% v/v fetal bovine serum. It is not a serum-free epithelial cell culture medium.

Specifications

Product Name	XR Epithelial Cell Growth Medium Kit
Product Type	Low-serum primary epithelial cell growth medium kit
Kit Reference	XREpiGM-500-A
Basal Medium	Epi Basal Medium, 480 mL
Supplement Options	EpiGM-M Supplement, 20 mL EpiGM-B Supplement, 20 mL
Supplement References	XREpiGM-500-(M)B XREpiGM-500-(B)B
Serum Content	2% v/v fetal bovine serum in the complete formulation
Growth Components	Recombinant human epidermal growth factor and transferrin
pH	7.2–7.4
Endotoxin	≤0.1 EU/mL
Filtration	<0.2 μm
Bacteria Detection	Not detected
Fungi Detection	Not detected
Antibiotics	Not included
Antifungal Agents	Not included
Basal Medium Storage	2–8°C, protected from light
Supplement Storage	-20°C, protected from light
Prepared Medium Storage	2–8°C for up to 4 weeks
Shelf Life	12 months
Research Grade	For Research Use Only

Medium Selection Guide

Select the supplement according to the epithelial cell type being cultured. The basal medium is combined with either EpiGM-B or EpiGM-M to create the appropriate complete primary epithelial cell growth medium.

Complete Medium Configuration	Recommended Cell Types
Epi Basal Medium + EpiGM-B	Retinal pigment epithelial cells
Epi Basal Medium + EpiGM-M	Bronchial epithelial cells, alveolar epithelial cells, mammary epithelial cells, corneal epithelial cells, conjunctival epithelial cells, renal epithelial cells, renal proximal tubule epithelial cells, sebaceous gland cells and prostate epithelial cells

Features

Low-serum epithelial cell culture medium containing 2% v/v fetal bovine serum
Designed for primary epithelial cell attachment, spreading, survival and expansion
Contains selected epithelial cell nutrients for balanced culture support
Contains recombinant human epidermal growth factor to support epithelial cell proliferation
Contains transferrin to support iron transport and cellular metabolism
Helps reduce premature senescence during primary epithelial cell culture
Designed to maintain epithelial cell phenotype and key cellular functions
Two supplement options for retinal pigment epithelial cells and other tissue-derived epithelial cells
Low endotoxin specification of ≤0.1 EU/mL
Filtered through a <0.2 μm filtration process
Supplied without antibiotics or antifungal agents for flexible experimental control
Convenient basal medium and supplement format for complete or partial preparation

Culture Medium Preparation

Complete 500 mL Preparation

Thaw the appropriate EpiGM supplement at 4°C overnight or at room temperature for 1–2 hours. Add the supplement to the Epi basal medium and mix thoroughly to prepare the complete epithelial cell culture medium.

Partial Preparation

For experiments that do not require the entire kit at one time, aliquot the supplement and prepare the medium using the following ratio:

EpiGM Supplement	4 mL
Epi Basal Medium	96 mL
Final Volume	100 mL

Store the prepared complete medium at 2–8°C and use within four weeks.

Optional Antibiotic Addition

The product does not contain antibiotics or antifungal agents. When required by the experimental protocol, an antibiotic solution may be added at 0.5–1% of the final culture volume.

Recommended Culture Conditions

Culture Stage	Recommended Seeding Density	Medium Change	Recommended Passage Point
Rapid Cell Growth	10,000–15,000 cells/cm²	Change medium every other day	70–80% confluence
Routine Passage	5,000–8,000 cells/cm²	Change medium every other day	70–80% confluence

The optimal seeding density may vary according to cell source, donor, passage number, culture vessel, coating system and experimental objective. Researchers should confirm the appropriate conditions for their specific primary epithelial cell model.

Applications

XR Epithelial Cell Growth Medium Kit supports research workflows that require controlled culture and expansion of primary epithelial cells from multiple tissue sources. Its low-serum formulation and tissue-oriented supplement options make it suitable for epithelial cell isolation, routine propagation, morphology monitoring and downstream functional studies.

Primary Epithelial Cell Culture and Expansion

Establishment of primary epithelial cell cultures from tissue-derived samples
Primary human epithelial cell attachment and spreading studies
Routine propagation and expansion of epithelial cell populations
Evaluation of epithelial cell morphology during in vitro culture
Preparation of epithelial cells for downstream molecular and cellular assays

Airway and Pulmonary Epithelial Cell Culture

Bronchial epithelial cell culture and expansion
Airway epithelial cell growth studies
Alveolar epithelial cell culture research
Respiratory epithelial cell morphology and proliferation analysis
In vitro studies involving respiratory tissue-derived epithelial cells

Ocular Epithelial Cell Research

Retinal pigment epithelial cell culture using EpiGM-B supplement
RPE cell attachment, maintenance and expansion studies
Corneal epithelial cell culture using EpiGM-M supplement
Conjunctival epithelial cell growth and morphology studies
Research involving ocular surface and retinal epithelial cell models

Renal Epithelial Cell Culture

Renal epithelial cell culture and proliferation research
Kidney epithelial cell maintenance in vitro
Renal proximal tubule epithelial cell culture
Evaluation of renal epithelial cell morphology and growth behavior
Preparation of kidney-derived epithelial cells for downstream assays

Mammary and Prostate Epithelial Cell Research

Primary mammary epithelial cell culture and expansion
Mammary epithelial cell proliferation studies
Primary prostate epithelial cell culture
Prostate epithelial cell morphology and phenotype research
In vitro studies involving glandular epithelial cell populations

Sebaceous Gland Cell Culture

Culture and expansion of sebaceous gland-derived cells
Evaluation of sebaceous cell morphology and proliferation
Preparation of sebaceous gland cells for cellular research workflows

Epithelial Cell Phenotype and Function Studies

Evaluation of epithelial morphology during primary cell expansion
Studies of cell attachment and spreading
Assessment of epithelial cell proliferation and survival
Research involving maintenance of epithelial characteristics in vitro
Comparison of culture conditions for tissue-derived epithelial cells

Why Choose XR Epithelial Cell Growth Medium Kit?

Successful primary epithelial cell culture requires more than basic nutrient supplementation. Cells isolated from tissues may attach inefficiently, expand slowly or undergo early senescence when culture conditions are not adapted to epithelial cell requirements.

XR Epithelial Cell Growth Medium Kit combines an optimized basal medium with selected epithelial supplements, recombinant human epidermal growth factor, transferrin and a controlled 2% serum concentration. This formulation is designed to provide sufficient growth support without relying on a conventional high-serum culture system.

The availability of separate EpiGM-B and EpiGM-M supplements allows researchers to select a culture configuration according to the epithelial cell source. This makes the system suitable for retinal pigment epithelial cells as well as respiratory, ocular surface, renal, mammary, prostate and sebaceous gland-derived epithelial cells.

Designed specifically for primary epithelial cell culture rather than general-purpose cell maintenance
Supports cell attachment, spreading, survival and proliferation
Low-serum formulation containing 2% v/v fetal bovine serum
Contains recombinant human EGF and transferrin
Supports multiple tissue-derived epithelial cell types
Includes separate supplement options for RPE cells and other epithelial cells
Low endotoxin and <0.2 μm filtered formulation
Supplied without antibiotics or antifungal agents
Suitable for complete-bottle or partial-volume preparation

FAQ

What is XR Epithelial Cell Growth Medium Kit used for?

XR Epithelial Cell Growth Medium Kit is used for the in vitro culture and expansion of primary epithelial cells. It is designed to support cell attachment, spreading, survival, proliferation and epithelial phenotype maintenance.

What is primary epithelial cell growth medium?

Primary epithelial cell growth medium is a specialized culture formulation that provides nutrients and growth-supporting components for epithelial cells isolated from tissues. Unlike a general-purpose medium, it is designed around the attachment, proliferation and phenotype requirements of primary epithelial cells.

Is XR Epithelial Cell Growth Medium serum-free?

No. This product is a low-serum epithelial cell culture medium containing 2% v/v fetal bovine serum. It should not be marketed or described as serum-free.

Which supplement should be used for retinal pigment epithelial cells?

Retinal pigment epithelial cells should be cultured using Epi basal medium combined with EpiGM-B supplement.

Which supplement should be used for bronchial or alveolar epithelial cells?

Bronchial and alveolar epithelial cells should be cultured using Epi basal medium combined with EpiGM-M supplement.

Can the medium be used for corneal epithelial cell culture?

Yes. Epi basal medium combined with EpiGM-M supplement is intended for corneal epithelial cell culture. The same configuration can also be used for conjunctival epithelial cells.

Can the medium be used for renal proximal tubule epithelial cells?

Yes. The Epi basal medium and EpiGM-M supplement configuration is suitable for renal epithelial cells and renal proximal tubule epithelial cells.

Can the medium be used for mammary and prostate epithelial cells?

Yes. EpiGM-M complete medium is intended for primary mammary epithelial cells and prostate epithelial cells, as well as several other tissue-derived epithelial cell types.

Does the medium contain epidermal growth factor?

Yes. The formulation contains recombinant human epidermal growth factor to support epithelial cell proliferation and culture performance.

Does the medium contain transferrin?

Yes. Transferrin is included as part of the epithelial cell growth-supporting formulation.

Does XR Epithelial Cell Growth Medium contain antibiotics?

No. The supplied product does not contain antibiotics or antifungal agents. Laboratories may add an antibiotic solution at 0.5–1% when required by their protocol.

How should the EpiGM supplement be thawed?

Thaw the supplement at 4°C overnight or at room temperature for approximately 1–2 hours before mixing it with the basal medium.

What is the recommended mixing ratio for partial medium preparation?

For partial preparation, combine 4 mL of EpiGM supplement with 96 mL of Epi basal medium to prepare 100 mL of complete epithelial cell growth medium.

How long can the prepared complete medium be stored?

The prepared complete medium can be stored at 2–8°C for up to four weeks.

What seeding density is recommended for rapid epithelial cell growth?

For rapid growth, a seeding density of approximately 10,000–15,000 cells/cm² is recommended. Change the medium every other day and passage the cells at approximately 70–80% confluence.

What seeding density is recommended for routine passage?

For routine passage, a seeding density of approximately 5,000–8,000 cells/cm² is recommended. Change the medium every other day until the culture reaches approximately 70–80% confluence.

What are the storage conditions for the kit components?

Store the Epi basal medium at 2–8°C protected from light. Store EpiGM-M and EpiGM-B supplements at -20°C protected from light.

What is the endotoxin specification?

The endotoxin specification is ≤0.1 EU/mL.

Is this product intended for clinical use?

No. XR Epithelial Cell Growth Medium Kit is intended for research use only and is not intended for diagnostic, therapeutic or clinical applications.

XR Human Epithelial Cell Medium Kit