Description

Transfection Reagent is a next-generation cationic lipid-based delivery system optimized for efficient transfection of plasmid DNA, messenger RNA (mRNA), and small interfering RNA (siRNA) into a broad range of eukaryotic cells. Its proprietary lipid nanoparticle formulation ensures strong gene expression results while minimizing cytotoxicity—making it ideal for both routine and advanced applications, including CRISPR and RNA-based therapeutics research.

Features

High-Efficiency Transfection of mRNA: Comparable in performance to Thermo Fisher’s Lipofectamine™ MessengerMAX™, Biofargo achieves excellent mRNA delivery with minimal toxicity—ideal for mRNA vaccines, gene editing, and protein expression studies.
Versatile Nucleic Acid Compatibility: Enables efficient transfection of plasmid DNA, mRNA, siRNA, and other nucleic acid formats using a unified protocol.
Superior Cell Viability: Reduces cellular stress and preserves morphology, ensuring reliable downstream analysis.
Compatible with Serum and Antibiotics: No need to remove serum or antibiotics, simplifying workflows.
Proven in Difficult-to-Transfect Cell Lines: Effective with primary cells, stem cells, suspension cells, and commonly used lines like HEK293, HeLa, and CHO.

Applications

mRNA transfection and expression
siRNA-mediated gene knockdown
Gene editing (CRISPR/Cas9)
Protein production
Functional genomics and cell engineering

siRNA Transfection Assay

1. Primary rat cardiomyocytes: siRNA knockdown efficiency of 82%.

2. BMDM cells: siRNA knockdown efficiency of 60% with no detectable cytotoxicity.

3. RAW264.7 cells: siRNA knockdown efficiency of 70%.

4. U87MG cells: siRNA knockdown efficiency of 85%, accompanied by significant inhibition of cell proliferation.

Reference for Cell Transfection Efficiency

Cell Name	Knockdown Efficiency
HeLa	85%
U-2 OS	85%
HEK293	85%
HCT-116	85%
BHK-21	85%
COS-7	85%
HT-1080	85%
NCI-H441	85%
U-87 MG	85%
Glial	80%
Primary Rat Cortical Cells	80%
Primary Rat Hippocampal	80%
HepG2	80%
A549	80%
K-562	80%
MCF7	80%
Jurkat	80%
THP-1	80%
L-929	80%
3T3	80%
Huh7	80%
NK-92	80%
Raji	80%
Rat Cardiomyocytes	80%
Saos-2	80%
U-937	80%
SW480	80%
L6	80%
PC-3	80%
4T1	80%
Raw264.7	70%
HUVEC	70%
Human T Cells	70%
SH-SY5Y	70%
Mouse Embryonic StemCells	60%
PBMC	60%
BMDM	60%
Mesenchymal Stem Cell (hMSC)	60%

FAQ

Q: Can I deliver single-stranded oligonucleotides with Transfection Reagent?
A: If delivering a single-stranded oligonucleotide alone, it's recommended to use Lipofectamine RNAiMAX Transfection Reagent. However, Transfection Reagent can be used for genome editing when co-delivering Cas9 mRNA, sgRNA, and a donor template (ssDNA or dsDNA).

Q: How do I use Transfection Reagent to deliver both Cas9 mRNA and IVT gRNA together?
A: Transfection Reagent supports co-delivery of Cas9 mRNA with single or multiple gRNAs, providing high transfection efficiency. It is compatible with cleavage detection or selection kits for downstream mutation analysis.

Q: Which cell types can be used with Transfection Reagent?
A: Transfection Reagent shows low cytotoxicity and high efficiency in various cell types, including primary and stem cells, as well as those that are typically difficult to transfect.

Q: When should I use Transfection Reagent over other reagents?
A: Use Transfection Reagent for CRISPR applications requiring the co-delivery of mRNA and gRNA, along with short donor DNA templates. Its flexibility makes it ideal for HDR-based genome editing.

Q: What is Transfection Reagent?
A: Transfection Reagent is an animal-origin-free reagent formulated for delivery of mRNA, small RNA (e.g., gRNA, siRNA, miRNA), and short donor DNA (0.5–1 kb), making it highly suitable for CRISPR genome editing workflows.