Specification

Full name	TOP2A rabbit monoclonal antibody
Alternative names	50 μl/100 μl
Reactivity	rabbit monoclonal
Applications	WB, IHC
Host	Rabbit
Clone type	rabbit monoclonal
Target Background	This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]
Swissprot No	P11388
Gene Accession	7153
WB Predicted band size
WB Positive control	P291559-WB-1-P
WB Recommended dilution	174 kDa
IHC predicted cell location	Predicted cell location: Nucleus
IHC positive control	Positive control: Human colon cancer
IHC Recommed dilution	Recommended dilution: 50-100

Storage

-20°C, Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Avoid repeated freeze-thaw cycles.

WB PIC Description

P291559-WB-1-P

IHC PIC Description

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using P291559(TOP2A Antibody) at dilution 1/50

TOP2A rabbit monoclonal antibody