Description

This kit utilizes a unique lysis buffer combined with a specially optimized, efficient humic acid precipitation agent to extract genomic DNA from various microorganisms, including Gram-negative bacteria, Gram-positive bacteria, and fungi, present in diverse environmental samples such as soil, feces, fermentation products, water, pond sludge, marine sediment, and gut contents.

The grinding beads and homogenizer can effectively crush the microorganisms in the environmental samples to ensure the high efficiency and integrity of the extracted genomic DNA.

Genomic DNA extracted by this kit has few impurities and good integrity, and can be directly used in downstream experiments of molecular biology such as PCR, database building, and sequencing.

Kit Contents

Contents		GDP713 (96 preps)
DP713	Buffer SNA	80 mL
	Buffer SNE	80 mL
	HA Removal Buffer CN	30 mL
	Buffer LB4A	40 mL
	Buffer W2	160 mL
	Buffer RW	40 mL
	Buffer TB	15 mL
	Buffer TE	15 mL
	RNase A (10 mg/mL)	1 mL
	MagAttract Suspension BE (100 mg/mL)	2 × 1 mL
OSE-TH-B08	Envir-DNA Grinding Beads Tube	2 × 48 tubes / pack

Features

Quick and simple: Quickly remove humic acid in 1 minute.
High purity: The extracted DNA is of high purity and can be used directly in downstream experiments.
Wide application: Suitable for microbial genomic DNA extraction from many types of environmental samples.

Applications

Soil samples (common soil, high humic acid soil, lean soil, difficult soils)
Fecal samples (mouse, livestock, poultry, gut contents)
Water samples and fermentation broths
Pond sludge, lake sediment, deep-sea sediment
Air and water filter membranes
Plant, fungal, and tissue-associated environmental samples

Storage

The kit can be stored for 15 months at room temperature (15–30 °C) under dry conditions.

If precipitation occurs, preheat the solution in a 37 °C water bath for 10 minutes before use to dissolve the precipitate without affecting performance.

Notes

Newly collected samples usually yield higher DNA amounts. Refer to optimal preservation conditions for different sample types before sampling.
Excess DNA may inhibit downstream PCR reactions. If inhibition occurs, dilute the DNA template before use.
HA Removal Buffer CN should be pre-chilled at 2–8 °C before use to achieve optimal humic acid removal efficiency.
Two elution buffers are provided:
- Buffer TB: Does not contain EDTA
- Buffer TE: Contains EDTA (recommended for long-term storage, but EDTA may inhibit PCR and library construction)
Due to the complexity of environmental samples, extracted nucleic acid concentrations may be low and degradation may occur during long-term storage. It is recommended to perform downstream experiments as soon as possible after extraction.

Documents

INFO

TIANMicrobe Magnetic Envir-DNA Kit