Description

TBE Buffer (Tris-Borate-EDTA) 10× Solution is a concentrated electrophoresis buffer designed for high-resolution DNA analysis in both agarose gel and polyacrylamide gel electrophoresis applications. By simply diluting the concentrate with distilled or deionized water, researchers can rapidly prepare a consistent 1× working solution while eliminating the variability associated with manual buffer preparation.

TBE buffer is widely used for PCR product analysis, DNA fragment sizing, molecular cloning workflows, restriction digestion verification, and nucleic acid quality assessment. Compared with TAE buffer, TBE provides superior separation of DNA fragments ranging from approximately 100 bp to 3 kb, producing sharper bands and improved resolution for routine molecular biology applications.

Its higher buffering capacity also makes TBE an excellent choice for extended electrophoresis runs and high-voltage applications, helping maintain stable pH and consistent migration performance throughout the experiment. Whether used for routine PCR verification or demanding analytical electrophoresis workflows, TBE Buffer 10× Solution delivers reliable and reproducible results for research laboratories.

Specification

Parameter	Specification
Total Product Size	4 L
Individual Container Size	4 L
Number of Containers	1
Shipping Conditions	Room Temperature (RT)
UNSPSC Code	41105323
UNSPSC Category	Electrophoresis Buffers
Grade	Biotech
Dangerous Goods (DG)	No
Storage	18–25°C
Sterile	No
Stock Status	Usually in Stock

Features

Superior Resolution for Small DNA Fragments

Provides excellent separation of DNA fragments between approximately 0.1 and 3 kb, making it ideal for PCR product analysis, cloning verification, and restriction fragment evaluation.
Optimized for Agarose and PAGE Electrophoresis

Suitable for both agarose gel electrophoresis and polyacrylamide gel electrophoresis workflows, offering versatility across a wide range of molecular biology applications.
High Buffering Capacity

Maintains stable electrophoretic conditions during longer run times and minimizes pH drift compared with lower-capacity buffer systems.
Supports High-Voltage Electrophoresis

Particularly suitable for electrophoresis performed at voltages above 150 V, where stable conductivity and buffering performance are critical.
Convenient 10× Concentrated Format

Dilute directly to a 1× working solution, reducing preparation time while ensuring reproducible buffer composition between experiments.
Consistent Performance for Routine Laboratory Work

Manufactured to Biotechnology Grade standards with controlled pH specifications to support reproducible DNA electrophoresis results.
Ideal for Frequent Electrophoresis Workflows

Well suited for research laboratories, university facilities, biotechnology companies, and core laboratories performing routine nucleic acid analysis.

Applications

PCR Product Verification

Confirm amplification success, evaluate specificity, and accurately determine amplicon size through high-resolution gel electrophoresis.
DNA Fragment Analysis

Separate and analyze DNA fragments generated from PCR, restriction digestion, cloning, or molecular characterization workflows.
Molecular Cloning Workflows

Verify recombinant constructs, screen transformants, and assess plasmid digestion products prior to downstream applications.
Polyacrylamide Gel Electrophoresis (PAGE)

Suitable for nucleic acid PAGE applications requiring high-resolution fragment separation.
High-Voltage Electrophoresis

Recommended for rapid electrophoresis protocols utilizing elevated voltages while maintaining reliable buffering performance.
DNA Quality Assessment

Evaluate nucleic acid integrity, purity, and fragment distribution during routine molecular biology workflows.
Academic and Research Laboratories

Ideal for molecular biology, genetics, biotechnology, microbiology, synthetic biology, and life science research laboratories conducting routine DNA electrophoresis.

Documents

INFO

COA

SDS

TBE Buffer (Tris-Borate-EDTA), 10x Solution - Biofargo