Specification

Package	100 RXNS
Full name	SpeedyCut SfiI
Applications	Fast enzymatic digestion
Summary	SpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.
Features	All SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.
Composition	100 ul enzyme, 10X SpeedyOne buffer
Recognition Site	5'…-GGCCNNNN↓NGGCC-…3' 3'…-CCGGN↑NNNNCCGG-…5'
Reaction Buffer	10X SpeedyOne Buffer
Recommended Reaction Temperature	50°C
Unit Definition	At 50°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg p615 DNA in 15 minutes.
Methylation Effects	Dam: Methylation does not affect; Dcm: Sequences overlap completely, cleavage blocked; CpG: Completely overlapping sequences, cleavage blocked; EcoKI: Methylation does not affect; EcoBI: Methylation does not affect.
Thermal Inactivation	Do not heat inactivate, use phenol chloroform extraction or column purification
Component	100 ul enzyme, 10X SpeedyOne buffer

Storage

-20°C