Description

The StarLighter Fluorometer S96 is a compact fluorescence reader designed for quantitative detection of DNA, RNA, microRNA, and proteins. It processes up to 96 samples using a 0.3 mL 96-well flat-bottom black ELISA plate and requires 1–20 μL of sample per well.

The instrument uses two fluorescence channels, LED light sources, a SiPMT detector, and top-reading detection. A complete 96-well plate can be read in 55 seconds. Supported test items include dsDNA, ssDNA, RNA, microRNA, proteins, and fluorescence intensity measurements.

Samples are combined with the appropriate working solution to a total volume of 200 μL per well. The instrument is operated through PC software that supports protocol creation, sample and well configuration, result review, data import, and Excel-format data export.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Specifications

Parameter	Specification
Product Name	StarLighter Fluorometer S96
Model	FS-M2005
Sample Volume	1–20 μL
Total Reaction Volume	200 μL per well
Sample Throughput	96
Compatible Consumable	0.3 mL 96-well flat-bottom black ELISA plate
Compatible Plate Dimensions	128 × 86 × 15 mm
Maximum Consumable Height	15 mm
Read Time	55 seconds per whole plate
Light Source	LED
Fluorescence Channels	2
Blue Excitation Wavelength	457–483 nm
Red Excitation Wavelength	622–638 nm
Green Emission Wavelength	520–540 nm
Red Emission Wavelength	672–712 nm
Detector	SiPMT
Detection Method	Top detection
Sample Types	dsDNA, ssDNA, RNA, microRNA, and proteins
dsDNA Sensitivity	0.05 ng/μL
ssDNA Sensitivity	0.1 ng/μL
RNA Sensitivity	0.5 ng/μL
microRNA Sensitivity	0.1 ng/μL
dsDNA HS Detection Range	0.01–100 ng/μL
dsDNA BR Detection Range	1–1000 ng/μL
RNA Detection Range	0.25–100 ng/μL
RNA BR Detection Range	1–1000 ng/μL
Calibration	2 points
Dynamic Range	5 orders of magnitude
Communication Interface	Bluetooth and serial port
Power Supply	DC 24 V, 2.5 A
Maximum Power	60 W
Operating Environment	15–30°C, <75% RH, indoor use only
Dimensions	274 × 180 × 106 mm
Weight	2.7 kg

Features

96-Sample Throughput: The instrument processes up to 96 samples using a 0.3 mL 96-well flat-bottom black ELISA plate.
Fast Whole-Plate Reading: A complete 96-well plate can be read in 55 seconds.
Low Sample Input: Each reaction requires 1–20 μL of sample, with working solution added to a total volume of 200 μL per well.
Two Fluorescence Channels: The instrument provides blue and red excitation channels with green and red emission detection.
Multiple Sample Types: Supported sample types include dsDNA, ssDNA, RNA, microRNA, and proteins.
Top-Reading Detection: Fluorescence is measured from the top of the microplate using a SiPMT detector.
Two-Point Calibration: Quantitative measurements are generated using two-point calibration.
Five-Order Dynamic Range: The instrument provides a dynamic range of five orders of magnitude.
PC Software Operation: The software supports protocol creation, well selection, sample information entry, experiment control, result review, data import, and data export.
Bilingual Software: The software interface supports Chinese and English.
Excel Data Export: Saved experimental results can be exported in Excel format.

Applications

dsDNA Quantification: Quantitative fluorescence measurement of double-stranded DNA samples.
ssDNA Quantification: Quantitative fluorescence measurement of single-stranded DNA samples.
RNA Quantification: Quantitative fluorescence measurement of RNA samples.
microRNA Quantification: Quantitative fluorescence measurement of microRNA samples.
Protein Quantification: Quantitative fluorescence measurement of protein samples.
RFU Measurement: Measurement of fluorescence intensity without a standard curve.

Frequently Asked Questions (FAQs)

Common questions about the StarLighter Fluorometer S96.

What can the StarLighter Fluorometer S96 measure?

The instrument is designed for quantitative fluorescence detection of dsDNA, ssDNA, RNA, microRNA, and proteins. It can also measure fluorescence intensity in RFU mode.

How many samples can be measured in one run?

The instrument supports up to 96 samples using a 0.3 mL 96-well flat-bottom black ELISA plate.

How long does it take to read a complete plate?

A complete 96-well plate can be read in 55 seconds.

How much sample is required per well?

Each well requires 1–20 μL of sample. Add 180–199 μL of working solution so that the total volume in each well is 200 μL.

What microplate is compatible with the instrument?

Use a 0.3 mL 96-well flat-bottom black ELISA plate measuring 128 × 86 × 15 mm. The height of the consumable must not exceed 15 mm.

Does the instrument require a computer?

Yes. The instrument must be connected to a computer using the supplied USB cable. The PC software is used to create protocols, configure wells, run experiments, review results, and export data.

How long should the instrument stabilize after power-on?

Allow the instrument to stabilize for 15 minutes after power-on before use.

Is the instrument intended for diagnostic use?

No. The instrument is for research use only and is not intended for diagnostic or therapeutic procedures.

Documents

Product User Manual

S96 96-Well Fluorometer for DNA, RNA and Protein Quantification