Note:Suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and hybridomas, require supplementation, commonly with 10% Fetal Bovine Serum (FBS).

Product Description

RPMI-1640 Medium was developed by Moore et al., at Roswell Park Memorial Institute. It was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI-1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and hybridomas. RPMI-1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

Specifications

Volume	500mL
Supplementation	Need additional
Glutamine	L-Glutamine
Phenol Red	Phenol Red
Antibiotics	No Antibiotics
Sodium Pyruvate	No Sodium Pyruvate
Sodium Bicarbonate	Sodium Bicarbonate
HEPES Buffer	No HEPES
pH	7.0-7.6
Osmolality	265~305 mOsmol/kg
Endotoxin	<0.3 EU/mL

Formulation

Components (mg/L)

Amino Acids

Glycine	10
L-Arginine (free base)	200
L-Asparagine (anhyd.)	50
L-Aspartic Acid	20
L-Cystine•2HCl	65
L-Glutamic Acid	20
L-Glutamine	300
L-Histidine (free base)	15
L-Hydroxyproline	20
L-Isoleucine	50
L-Leucine	50
L-Lysine•HCl	40
L-Methionine	15
L-Phenylalanine	15
L-Proline	20
L-Serine	30
L-Threonine	20
L-Tryptophan	5
L-Tyrosine•2Na•2H₂O	29
L-Valine	20

Vitamins

D-Biotin	0.2
Choline chloride	3
Folic Acid	1
myo-Inositol	35
Niacinamide	1
p-Amino Benzoic Acid	1
D-Pantothenic Acid•1/2 Ca	0.25
Pyridoxal•HCl	1
Riboflavin	0.2
Thiamine•HCl	1
Vitamin B-12	0.005

Inorganic Salts

Ca(NO₃)₂•4H₂O	100
MgSO₄(anhyd.)	48.84
KCl	400
NaHCO₃	2000
NaCl	6000
NaH₂PO₄(anhyd.)	800

Other

D-Glucose	2000
Glutathione (reduced)	1
Phenol Red•Na	5

Storage

Stored at 2℃~8℃, away from light, the shelf life is 12 months.

Notes

Avoid repeated freezing and thawing.
Pay attention to operate in an aseptic condition.

Document

RPMI-1640 Medium-Biosci™