Description

SHENTEK^® Residual SV40LTA & E1A DNA Quantitation Kit (2G) is used to quantitate residual SV40LTA & E1A host cell DNA, such as HEK293T cell. This sv40 large t antigen kit uses duplex real-time PCR technique to performs rapid, specific, and reliable quantitation assay at the level of 101 copies/μL. For extraction information, please refer to the SHENTEK^® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191)

Introduction

Components	Shipping Condition	Unit Size	Detection Method
SV40LTA & E1A linear DNA Control	-20℃	100 Reactions	Probe-basedq PCR
SV40LTA & E1A non-linear DNA Control	-20℃
qPCR Reaction Buffer	-20℃, protect from light
SV40LTA & E1A Primer & Probe MIX
IPC MIX
DNA Dilution Buffer (DDB)	-20℃

Specification

Range	SV40LTA: 4.67x10¹-4.67x10⁶ pg/μL, R²=0.99974, E=97.5%
	E1A: 4.97x10¹-4.97x10⁶ pg/μL, R²=0.99946, E=98.3%
Accuracy	SV40LTA: Recovery = 105.2%-110.1%, CV<30%
	E1A: Recovery = 115.8%-124.5%, CV<30%
LOQ	SV40LTA: 9.34 copies/μL
	E1A: 49.7 copies/μL
LOD	SV40LTA: 0.934 copies/μL
	E1A: 0.497 copies/μL
Precision	SV40LTA: 1.7%-9.0% （<30%）
	E1A: 1.9%-9.4%（<30%）
Robustness	Freeze-thaw stability over 5 cycles
	Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments

Documents

User Guide

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SHENTEK™ Residual Host Cell DNA Sample Preparation Kit

Residual SV40 LTA/E1A DNA Quantitation Kit (2G) SHENTEK™